Addgene

Showing: 2721 - 2740 of 3797 results

Results per page:

Sort results by:

pLX317-MBNL1

Plasmid

#115443

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL1 (MBNL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mouse a2b full length

Plasmid

#217812

Purpose

Full length mouse integrin a2b expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGA2B (Itga2b Mouse)

Use

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2-sgCTRL

Plasmid

#209750

Purpose

Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

Cas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL_SRSF2_P95H_mCherry

Plasmid

#84023

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95H

Promoter

PGK

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX317-CELF4

Plasmid

#115445

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

CELF4 (CELF4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHes1(2.5k)-luc

Plasmid

#43806

Depositor

Ryoichiro Kageyama

Article

Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

Insert

Hes1 Promoter (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter

Promoter

Hes1 Promoter

Available since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC23A2

Plasmid

#132025

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC23A2 (SLC23A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
LaminA-ECFP-SNAP

Plasmid

#187965

Purpose

expresses LaminA with C-terminal tags ECFP and SNAP

Depositor

Markus Wahl

Article

Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

Insert

LaminA (LMNA Human)

Use

Tags

ECFP-SNAP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Gfp-zf-vasa

Plasmid

#167323

Purpose

Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

Depositor

Goro Yoshizaki

Article

Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

Insert

zf vasa 3'UTRs (ddx4 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-NLS-actin-R62D

Plasmid

#118381

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Tags

Expression

Mutation

NLS and R62D

Promoter

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 (-) hYAP 1-2alpha

Plasmid

#124143

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Marius Sudol, Megan Finch-Edmondson

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean3-Bcl-XL-s2193

Plasmid

#166749

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-XL (BCL2L1 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Human ferritin

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB-Halo,IRES-eGFP

Plasmid

#164519

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ-hDDX5/17

Plasmid

#71307

Purpose

Inducible shRNA knockdown of both human DDX5 and DDX17 genes

Depositor

Allan Brasier

Article

Yang et al Mol Cell Proteomics. 2015 Oct;14(10):270

Insert

DDX5/17 shRNA (DDX17 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC23A1

Plasmid

#132013

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC23A1 (SLC23A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
TRE-UBC-hCD59-T2A-GFP

Plasmid

#205460

Purpose

lentiviral plasmid for expression of human CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD59 (CD59 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FCM101

Plasmid

#194298

Purpose

E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6

Depositor

Shohei Koide

Article

Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:

Insert

FCM101

Use

Tags

His tag, FLAG tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mouse b5 full length

Plasmid

#217815

Purpose

Full length mouse integrin b5 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGB5 (Itgb5 Mouse)

Use

Tags

P2A-mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-puro-N-Flag-h-SNRNP40

Plasmid

#134254

Purpose

Lentivector encoding Flag-tagged human SNRNP40

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

SNRP40 (SNRNP40 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL_SRSF2_P95L_mCherry

Plasmid

#84022

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95L

Promoter

PGK

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only