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Plasmid#210360PurposeExpresses TMEM106B fused with 2Strep in mammalian cellsDepositorInsertTMEM106B (TMEM106B Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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SGTA-2Strep
Plasmid#210353PurposeExpresses SGTA fused with 2Strep in mammalian cellsDepositorInsertSGTA (SGTA Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT348
Plasmid#204515PurposeBacterial expression of CEC-5 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertC-terminal fragment of cec-5 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB312
Plasmid#203569PurposeTU for the expression of Nanoluciferase (Nluc) under the PgpdA promoter.DepositorInsertPgpdA:Nluc:Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT353
Plasmid#204506PurposeIn vitro transcription of RNA probes for in situ hybridization of CeRep55 lncRNAs in nematode (after linearization with NotI and BglI)DepositorInsertCeRep55 tandem repeats from Y73B3A (C. elegans)
UseOtherTagsExpressionMutationPromoterdouble-T7Available sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT334
Plasmid#204516PurposeBacterial expression of COH-3 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertC-terminal fragment of coh-3 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT203
Plasmid#204512PurposeBacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertC04F12.1 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT517
Plasmid#204518PurposeBacterial expression of CEC-4 with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertcec-4 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT521
Plasmid#204520PurposeBacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertN-terminal fragment of cec-5 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT342
Plasmid#204514PurposeBacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertN-terminal fragment of syp-1 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain
Plasmid#183387PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertredesigned Caspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationANR261ENP, D315H, M318K, T352KPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-mouse-Caspase-1-H340D
Plasmid#183362PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1 H340D (Casp1 Mouse)
UseRetroviralTagsMycExpressionMutationH340DPromoterMESVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T
Plasmid#183364PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4a (CASP4 Canis lupus familiaris)
UseRetroviralTagsMycExpressionMutationH342D_K345M_K379TPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4b
Plasmid#183365PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4b (CASP4 Canis lupus familiaris)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4a
Plasmid#183363PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4a (CASP4 Canis lupus familiaris)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJH2198
Plasmid#179773PurposePrgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFPDepositorInsertNLF-1::GFP (nlf-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrgef-1Available sinceMarch 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pRS426-CeLACE-1
Plasmid#174523PurposeExpress C.elegans LACE-1 in yeast cellsDepositorInsertlace-1 (C30F12.2 Nematode)
UseTags6xHisExpressionYeastMutationPromoterMET25Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only