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pMCSG49-AlleyCat7

Plasmid

#42591

Depositor

Ivan Korendovych

Article

Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

Insert

mutated C-terminal portion of calmodulin gene (M76-K148)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Isole…

Promoter

T7

Available since

March 21, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-MCHpr-GCaMP6f

Plasmid

#221039

Purpose

Expresses GCaMP6f under the rat pro-melanin-concentrating hormone promoter

Depositor

Yanhua Huang

Article

Guo et al Biol Psychiatry. 2022 Dec 1;92(11):880-8

Insert

GCaMP6f

Use

AAV; Rat targeting

Tags

Expression

Mammalian

Mutation

A317E

Promoter

Rat MCH

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

Plasmid

#170823

Purpose

PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d PPP2CA gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 MMP2 g1

Plasmid

#170824

Purpose

PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d MMP2 gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 MMP2 g2

Plasmid

#170825

Purpose

PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d MMP2 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g1

Plasmid

#170826

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2

Plasmid

#170827

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1

Plasmid

#170828

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

Plasmid

#170829

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG eGFP NLS

Plasmid

#170830

Purpose

Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1

Plasmid

#170822

Purpose

PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d PPP2CA gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E6

Plasmid

#213469

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cda-32aa linker-z…

Tags

No

Expression

Plant

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E7

Plasmid

#213470

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cdd-32aa linker-z…

Tags

No

Expression

Plant

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E8

Plasmid

#213471

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cdd_v106w-32aa li…

Tags

No

Expression

Plant

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-mCardinal-P2A-B19L Flp-in vector

Plasmid

#178519

Purpose

For making TRE-CB mouse line in Tigre locus

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCardinal-P2A-B19L

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-tTA

Plasmid

#178516

Purpose

AAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expression

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-mCre

Plasmid

#178515

Purpose

AAV vector for mCre recombinase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCre

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

Plasmid

#178518

Purpose

For making Rabies virus SAD B19 strain L cell line with hyPBase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

B19L-IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-L17-FZY2-S100N-T26I&T77I-UGI-Puro

Plasmid

#209645

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

L17-FZY2-S100N-T26I&T77I

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 mePOU-POU

Plasmid

#206392

Purpose

Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Use

Tags

6xHis

Expression

Bacterial

Mutation

POU DNA binding domain only

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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