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Plasmid#173756PurposeBinary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160aDepositorInsertCas9_sgRNA1/R160a_sgRNA2/R160a
UseCRISPRTagsExpressionMutationPromoterAtU6 &StU6Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDe_Cas9_KANA_R160b
Plasmid#173757PurposeBinary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160bDepositorInsertCas9_sgRNA1/R160b_sgRNA2/160b
UseCRISPRTagsExpressionMutationPromoterAtU6 &StU6Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDe_Cas9_KANA_R390a
Plasmid#173758PurposeBinary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390aDepositorInsertCas9_sgRNA1/390a_sgRNA2/390a
UseCRISPRTagsExpressionMutationPromoterAtU6 &StU6Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.037
Plasmid#184966PurposeExpress -Eco1 dead RT and ncRNA in yeasstDepositorInsertEco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
UseTagsExpressionYeastMutationHuman codon optimized RT, YXDD catalytic region m…PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.71
Plasmid#184983PurposeExpress -Eco1 RT/Cas9 fusion, using linker1DepositorInsertEco1RT-fusion_linker1-Cas9
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjSF1-hCD271-PGK-Neo
Plasmid#186171PurposeKnock-in gene targeting in marmoset cells at SF1 locusDepositorInserthCD271
UseMarmoset targetingTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ammB3
Plasmid#167815PurposeExpresses His6-tagged AmmB3 in E. coliDepositorInsertAmmB3
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ammC1
Plasmid#167816PurposeExpresses His6-tagged AmmC1 in E. coliDepositorInsertAmmC1
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pNB200
Plasmid#169111PurposeTESEC backbone for target expressionDepositorInsertlacZ (lacZ Escherichia coli K12 MG1655)
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB201
Plasmid#169112PurposeTESEC expression vector for Alr from M. tuberculosisDepositorInsertalr (alr Mycobacterium tuberculosis H37Rv)
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB202
Plasmid#169113PurposeTESEC expression vector for mEGFPDepositorInsertmEGFP
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-gp130dimer-stm2585
Plasmid#174395PurposeConstitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)DepositorInsertIL6ST cytosolic domain and sarA chimera
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585
Plasmid#174391PurposeS. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tagDepositorInsertstm2585
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-HO-G
Plasmid#164163PurposeExpresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminalDepositorInsertmEGFP A236K
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM4 (H578A)
Plasmid#154858PurposeExpresses N-terminally HA-tagged PfROM4 variant H578A (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM4 (PF3D7_0506900 Synthetic)
UseTagstriple HAExpressionMammalianMutationHistidine 578 changed to AlaninePromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PymeACR1
Plasmid#153031PurposePrasinophyte anion-conducting channelrhodopsin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertPymeACR1
UseTagsmCherryExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphD
Plasmid#128138PurposeThe cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP
Plasmid#124066PurposeE. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.DepositorInsertcytATL-R232Q-sfGFP
UseTagssfGFPExpressionBacterialMutationR232QPromotertrcAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-191084-gp160
Plasmid#123234PurposeMammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolateDepositorInsertHIV-1 (191084 B7-19) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only