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Plasmid#199320Purposemutated phosphorylation site (MLKL interaction permanent active)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 227 to Aspartic AcidPromoterAvailable sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[S227A]-FLAG
Plasmid#199318Purposemutated phosphorylation site (MLKL interaction inhibited)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 227 to AlaninePromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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GFP-LacI-HIPK2
Plasmid#179269PurposeExpresses GFP-LacI-HIPK2 fusion proteinDepositorInserthomeodomain interacting protein kinase 2 (HIPK2 Human)
UseTagsGFP-LacIExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 JIP3a
Plasmid#53457Purposeinteracts with MAPK signaling moduleDepositorInsertmitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[S199D][S227D]-FLAG
Plasmid#199324Purposedouble mutated phosphorylation site (kinase acitivity and MLKL interaction active)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 199 and 227 to Aspartic AcidPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[S199A][S227A]-FLAG
Plasmid#199323Purposedouble mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 199 and 227 to AlaninePromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAX-mScarlet-CIP4
Plasmid#178364PurposeExpresses CIP4 c-terminal labeled with mScarlet in mammalian cellsDepositorInsertCdc42 Interacting Protein 4 (TRIP10 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr-D435A/E437A
Plasmid#40829PurposeExpresses Flag, Myc tagged mutant Nbr in Drosophila cellsDepositorInsertNbr D435A/E437A (Nbr Fly)
UseTags3xFLAG and 6xMycExpressionInsectMutationD435A/E437A, part of the DEDD motif; mutations p…PromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLC-EGFP-RIP1
Plasmid#75163PurposeLentiCRISPR-EGFP with sgRNA targeting human RIPk1DepositorInsertRIPK1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pclbw-PAGFPomp25
Plasmid#58427PurposeMammalian expression of photoactivatable GFP targeted to the mitochondrial outer membraneDepositorInsertpaGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsPAGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25Promotercmv-bactinAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP
Plasmid#103815PurposeSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue lightDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEA02
Plasmid#172193PurposeSuicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattL
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEA03
Plasmid#172194PurposeSuicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattR
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
FCK-ss-Prl-Arch-GFP
Plasmid#22224PurposeLentiviral expression of Arch-GFP, with N-terminal MHC Class I antigen signal sequence (ss) and Prolactin signal sequence (Prl), for optogenetic inhibition.DepositorInsertss-Prl-Arch-GFP
UseLentiviralTagsGFP, Prl, and ssExpressionMammalianMutationPromoterCaMKIIAvailable sinceJan. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-DNMT3A PWWP
Plasmid#59696PurposeContains histone modification interacting domain (DNMT3A PWWP)DepositorInsertDNMT3A PWWP (Dnmt3a Mouse)
UseTagsExpressionBacterialMutationPromotertac promoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-CBX7 Chromo
Plasmid#59695PurposeContains histone modification interacting domain (CBX7 Chromo)DepositorInsertCBX7 Chromo (CBX7 Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-mCherry
Plasmid#103816PurposeSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue lightDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only