Addgene

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pEGFP-N3-TYK2

Plasmid

#139354

Purpose

Expression of TYK2 protein fused with GFP

Depositor

Sandra Pellegrini

Article

Ragimbeau et al J Biol Chem. 2001 Aug 17;276(33):30812-8

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBJ084_zACNE20_myl7_FR-GECO1c_P2A_CoChR_KGC_eGFP_ER2

Plasmid

#209282

Purpose

Expresses FR-GECO1c and CoChR in the zebrafish heart

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

FR-GECO1c_P2A_CoChR_KGC_eGFP_ER2

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

zACNE20_myl7

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP FRNK

Plasmid

#50518

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Insert

protein tyrosine kinase, mutated

Use

Tags

GFP

Expression

Mammalian

Mutation

truncated (NT2186-3268)

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
AAVS1-SAGFP

Plasmid

#127100

Purpose

SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.

Depositor

Adam Karpf

Insert

SA-GFP reporter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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8047_GFP-Kras2B_ires_puro

Plasmid

#64371

Purpose

This a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

none

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pOBCol2.3-GFPemd

Plasmid

#110210

Purpose

Labels cells in the late osteoblast lineage.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPemerald

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

2.3 kb rat type 1 collagen + first intron

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 WT

Plasmid

#74950

Purpose

mammalian expression of Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
p274 EF1a_EGFP_hH11

Plasmid

#164851

Purpose

EGFP-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hEFA6B

Plasmid

#206413

Purpose

Mammalian expression of hEFA6B

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

Insert

EFA6B (PSD4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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mTagGFP2-Clathrin-15

Plasmid

#56413

Purpose

Localization: Clathrin Vesicles, Excitation: 483, Emission: 506

Depositor

Michael Davidson

Insert

Clathrin (CLTB Human)

Use

Tags

mTagGFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCDNA3.1-5'dGFP3'

Plasmid

#26425

Depositor

Erin Schuman

Article

Sutton et al Science. 2004 Jun 25. 304(5679):1979-83.

Insert

5'dGFP3'

Use

Tags

CamKIIa 3'-UTR, CamKIIa 5'-UTR, and dGF…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
prNFL-EGFP

Plasmid

#132605

Purpose

Expresses EGFP-tagged rat NFL

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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3xEGFP-mMeikin

Plasmid

#174708

Purpose

Mammalian expression of mouse Meikin

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin (Meikin Mouse)

Use

Tags

3xEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN-mEGFP-C1

Plasmid

#110468

Purpose

retrovirus construct for stable expressing mEGFP tagged protein

Depositor

Richard Youle

Article

Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

Insert

mEGFP

Use

Retroviral; Retrovirus

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRvCAHS3-mEGFP

Plasmid

#205008

Purpose

The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between.

Depositor

Kazuharu Arakawa

Article

Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

Insert

mEGFP flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus

Use

Tardigrade expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSSec7-mGFPx3

Plasmid

#25449

Depositor

Benjamin Glick

Article

Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

Insert

Sec7 (SEC7 Budding Yeast)

Use

Tags

mGFPx3

Expression

Yeast

Mutation

GFP contains A206K for monomerization.

Promoter

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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Myo 19-GFP

Plasmid

#134988

Purpose

Expression of human Myo 19 in mammalian cells.

Depositor

Martin Bähler

Article

Oeding et al J Cell Sci. 2018 Sep 10;131(17). pii: jc

Insert

Myo 19 (MYO19 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-ermGFP

Plasmid

#27172

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 19

Plasmid

#134987

Purpose

Expression of human Myo 19 in mammalian cells.

Depositor

Martin Bähler

Article

Oeding et al J Cell Sci. 2018 Sep 10;131(17). pii: jc

Insert

Myo 19 (MYO19 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:GFP1–9

Plasmid

#108187

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components (GFP1-9) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Insert

GFP1-9

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

UBQ10:sXVE:

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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