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Plasmid#27795DepositorInsertmCerulean N1
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS
Plasmid#132404Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsExpressionMammalianMutationH133A; H1059APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST HP1 gamma
Plasmid#24076DepositorInsertHP1 gamma (CBX3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS
Plasmid#199346PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKeima-Red-N1
Plasmid#54597PurposeLocalization: N1 Cloning Vector, Excitation: 440, Emission: 620DepositorTypeEmpty backboneUseTagsmKeima-RedExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC014 - LwCas13a-msfGFP
Plasmid#91902PurposeExpresses active LwCas13a for mammalian RNA targetingDepositorInsertLwCas13a
UseCRISPRTagsNLS and msfGFP-NLS-3xHAExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3_MERS_D12
Plasmid#170448PurposeExpress MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.DepositorInsertMERS spike D12 (S MERS)
UseTagsExpressionMammalianMutationlast 12aa deletion in c-terminal tailPromoterCMVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
hKCNQ3(WT).ires.mCherry-pcDNA3
Plasmid#204362PurposeHeterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)DepositorInsertKCNQ3 (KCNQ3 Human)
UseTagsExpressionMammalianMutationPromoterCMV/T7 RNApolAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE-FLP
Plasmid#45978DepositorInsertflp
UseSynthetic BiologyTagsExpressionBacterialMutationflp driven by the constitutive promoter pE from p…PromoterpE (from phage P2)Available sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Tomato
Plasmid#214403PurposeExpresses human SOX10, OLIG2, NKX6.2 and tdTomatoDepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cntn1-Fc-His
Plasmid#72065PurposeExpresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertCntn1 (Cntn1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
GPR125-Tango
Plasmid#66313PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR125 (ADGRA3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUBC_hsPUM1-mCherry
Plasmid#107607PurposeTransient expression of Pumilio1, fused to mCherry in mammalian cells, under a hUBC promoterDepositorInserthsPUM1-mCherry
UseSynthetic BiologyTagsmCherryExpressionMammalianMutationPromoterhuman UBCAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
N1-mSin1.1-GFP
Plasmid#72907PurposeGFP-tagged mSin1 (isoform 1)DepositorInsertmSin1.1 (MAPKAP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M2
Plasmid#135583PurposeHigh eukaryotic protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB46 (CK2beta)
Plasmid#27085DepositorInsertCK2beta (CSNK2B Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
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p2T-CMV-EA-BE4max-NG-BlastR
Plasmid#152992PurposeHigh purity C-to-T base editorDepositorInsertBE4 H47E+S48A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTA7001-DEST
Plasmid#71745PurposeGATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducibleDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterGAL4Available sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
GPR64-Tango
Plasmid#66369PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR64 (ADGRG2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB_SBE4:mScarlet-I-NLS
Plasmid#154961Purposeencodes transcriptional reporter of SMAD2/3DepositorInsertmScarlet
UsePiggybacTagsNLS-PESTExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-TagRFP657
Plasmid#96939PurposeRetroviral plasmid containing TagRFP657 for labeling of cellsDepositorInsertTag Red Fluorescent Protein 657
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-HA-hUBE1L
Plasmid#12438DepositorInsertUBE1L (UBA7 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDS-RED-C1-Beclin1
Plasmid#24405DepositorInsertBeclin1 (BECN1 Human)
UseTagsDsRedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV
Plasmid#216164PurposeExpression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of functionDepositorInsertGaussia princeps luciferase (with double mutation to improve stability)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
R-pre-GFP
Plasmid#17274DepositorInsertR-pre
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD3
Plasmid#20185DepositorInsertNotch 3 Intracellular Domain (Notch3 Mouse)
UseTags3X FlagExpressionMammalianMutationContains murine Notch 3 Intracellular Domain (Met…PromoterCMVAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS327
Plasmid#51787Purposemulticopy (YEp) vector with a LYS2 marker and a 2-μm origin of replicationDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413_Csy4NLS
Plasmid#103139PurposeExpresses Csy4-NLS under TEF promoterDepositorInsertCsy4
UseTagsNLSExpressionYeastMutationPromoterTEFAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Hygro
Plasmid#127763PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}
Plasmid#201120PurposeRFP expression in cells under EF-1α promoter; Histone-bound, nuclearDepositorInsertTurboRFP; H2B-RFP
UseLentiviralTagsExpressionMutationNoPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i1 ONE-GO
Plasmid#189735PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 WT
Plasmid#12301DepositorInsertSTAT1 (STAT1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
N1-mSin1.2-GFP
Plasmid#72908PurposeGFP-tagged mSin1 (isoform 2)DepositorInsertTarget of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PITPbeta-GFP
Plasmid#58274Purposemammalian expression of GFP-PITP betaDepositorInsertPITP beta (PITPNB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-BbsI-chiRNA
Plasmid#45946PurposePlasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.DepositorInsertU6-BbsI-chiRNA
UseCRISPRTagsExpressionInsectMutationPromoterDm-snRNA:U6:96AbAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG_GAPDH in pCW57_tTA_Blast
Plasmid#154211PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDHDepositorInsertGAPDH (GAPDH Human)
UseLentiviralTags3X FLAGExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BAX_WT
Plasmid#82191PurposeGateway Donor vector containing BAX , part of the Target Accelerator Plasmid Collection.DepositorInsertBAX (BAX Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXM1
Plasmid#153136PurposeExpression of Flag tagged human FOXM1 in mammalian cellsDepositorInserthuman FOXM1 (FOXM1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Rab7
Plasmid#14436PurposeMammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.DepositorInsertRab 7 (RAB7A Canis familialis)
UseTagsmRFPExpressionMammalianMutation3 silent mutations near the C-terminusPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)
Plasmid#203908PurposeEncodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cellsDepositorInsertGal4UAS_dGFP_PGK_iRFP
UseTagsExpressionMammalianMutationPromoter5xUAS and PGKAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFD5
Plasmid#73914Purposemultiplex gRNA plasmidDepositorInsertdU6-3:tRNA:gRNA:tRNA:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF1344
Plasmid#143792PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFEZF2 (FEZF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hSyn-NCreIntN-T2A-mTagBFP2
Plasmid#160502PurposeExpresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterhSynAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2960
Plasmid#144436PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPGR (PGR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-C12orf66
Plasmid#87048Purposemammalian expression of C12orf66DepositorInsertC12orf66 (C12orf66 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA
Plasmid#105665Purposeexpresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationPromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF2944
Plasmid#144420PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSATB2 (SATB2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-CUL3
Plasmid#19893DepositorInsertcullin 3 (CUL3 Human)
UseTags3xmycExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF1872
Plasmid#144374PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBPTF (BPTF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only