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Plasmid#167564PurposeThe GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-gfap(Intron1/5'/Exon1-zebrafish)
Plasmid#39761DepositorInsertzebrafish gfap promoter- EGFP (gfap Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromotergfapAvailable sinceAug. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTYF-sGFAP-IRES-tdTomato
Plasmid#166920PurposeEmpty plasmid backbone; includes IRES-tdTomato and sGFAP promoterDepositorTypeEmpty backboneUseLentiviralTagstdTomatoExpressionMammalianMutationPromotersGFAPAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV+ plasmid with onboard mCherry cassette
Plasmid#169741PurposeCMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc
Plasmid#141102Purposebioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosisDepositorInsertSEP-Nanoluc
UseLentiviralTagsExpressionMutationPromoterEF1αAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hTimeless (pc4729)
Plasmid#216861PurposeMammalian expression vector encoding GFP tagged human TimelessDepositorInsertTimeless (TIMELESS Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.4Ch (pc2393)
Plasmid#211730PurposeExpresses MBD2 (aa236-414) mCherry tagged in insect cellsDepositorInsertMBD2.4 aa236-414
UseTagsmCherryExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.3Ch (pc2392)
Plasmid#211729PurposeExpresses MBD2 (aa222-414) mCherry tagged in insect cellsDepositorInsertMBD2.3 aa222-414
UseTagsmCherryExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.5R (pc2395)
Plasmid#211727PurposeExpresses MBD2 (aa153-221) RFP tagged in insect cellsDepositorInsertMBD2.5 aa153-221
UseTagsRFPExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.6R (pc2397)
Plasmid#211725PurposeExpresses MBD2 (aa153-235) RFP tagged in insect cellsDepositorInsertMBD2.6 aa153-235
UseTagsRFPExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.1mR (pc2391)
Plasmid#211723PurposeExpresses MBD2 (aa1-152) RFP tagged in insect cellsDepositorInsertMBD2.1 aa1-152
UseTagsRFPExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB_MBD2.2-GFP (pc2083)
Plasmid#211722PurposeExpresses MBD2 (aa153-414) GFP tagged in insect cellsDepositorInsertMBD2.2 aa153-414
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB_MBD2.1-GFP (pc2082)
Plasmid#211721PurposeExpresses MBD2 (aa1-152) GFP tagged in insect cellsDepositorInsertMBD2 aa1-152
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MeCP2.9Ch (pc2385)
Plasmid#211720PurposeExpresses MeCP2 (aa163-309) mCherry tagged in insect cellsDepositorInsertMeCP2 aa163-309
UseTagsmCherryExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MeCP2G.9 (pc1574)
Plasmid#211719PurposeExpresses MeCP2 (aa163-309) GFP tagged in insect cellsDepositorInsertMeCP2 aa163-309
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MeCP2G.8 (pc1573)
Plasmid#211718PurposeExpresses MeCP2 (aa310-492) GFP tagged in insect cellsDepositorInsertMeCP2 aa310-492
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB MBD2a-2-strep (pc1794)
Plasmid#211715PurposeExpresses fl. MBD2 2xStrep tagged in insect cellsDepositorInsertMBD2
UseTags2xStrepExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB MBD2a-C-GFP (pc1565)
Plasmid#211712PurposeExpresses fl. MBD2 GFP tagged in insect cellsDepositorInsertMBD2
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBD2.1R (pc2813)
Plasmid#211575PurposeExpresses MBD2 (aa1-152) RFP tagged in mammalian cellsDepositorInsertMBD2.1 aa1-152
UseTagsRFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENmRFPCNAL2 pc1054
Plasmid#167567PurposeThe fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-E2A-cyOFP1
Plasmid#141084Purposeprecursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-E2A-cyOFP1
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGK+ episome
Plasmid#170041PurposePGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGK-tetO2Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-EEA1
Plasmid#174451PurposeExpress EGFP with early endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with early endosome-targeting sequence
UseTagsearly endosomal antigen 1 (EEA1)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-EEA1
Plasmid#174452PurposeExpress mCherry with early endosome-targeting sequence in mammalian cellsDepositorInsertmCherry with early endosome-targeting sequence
UseTagsearly endosomal antigen 1 (EEA1)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM7-Halo-FRB
Plasmid#197058PurposeExpression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L plasmid
Plasmid#169732PurposeEqualizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-iRFP-EEA1
Plasmid#174453PurposeExpress iRFP with early endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with early endosome-targeting sequence
UseTagsearly endosomal antigen 1 (EEA1)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-LOx-IRES-EGFP
Plasmid#166877PurposeExpress the bacterial lactate oxidase (LOx) enzyme in mammalian cellsDepositorInsertLactate oxidase
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro WT ATG3
Plasmid#26926DepositorInsertautophagy-related 3 (yeast) (Atg3 Mouse)
UseRetroviralTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-H plasmid
Plasmid#169367PurposeEqualizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.DepositorInserttetR-P2A-eGFP-miR(FF3) target-miR(FF3)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-M plasmid
Plasmid#169731PurposeEqualizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.DepositorInserttetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV+ episome
Plasmid#169742PurposeCMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGK+ plasmid with onboard mCherry cassette
Plasmid#169745PurposePGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGK-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTYF-sGFAP-LOx-IRES-tdTomato
Plasmid#166919PurposeExpress the bacterial lactate oxidase (LOx) enzyme in astrocytesDepositorInsertLactate oxidase
UseLentiviralTagstdTomatoExpressionMammalianMutationPromotersGFAPAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV FHA-ATG12
Plasmid#27049DepositorInsertautophagy-related 12 (yeast) (Atg12 Mouse)
UseRetroviralTagsHA and flagExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-IRES-Antares
Plasmid#141085Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-EMCV IRES-Antares
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc
Plasmid#141086Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-EMCV IRES-SEPLuc
UseTagsExpressionMammalianMutationDeletion of amino acids 66 to 91 on the Luciferas…PromoterCMVAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc IRESv24
Plasmid#141087Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRESDepositorInsertAntares-T2A-puromycin-IRESv24-SEPLuc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-EKAR4
Plasmid#205513PurposeGenetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.DepositorInsertendo-EKAR4
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-Nb37
Plasmid#205514PurposeEndosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.DepositorInsertendo-Nb37
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only