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We narrowed to 34,759 results for: lat

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pMJB1-RPL1B-Cl

Plasmid

#160430

Purpose

Expresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoter

Depositor

Gary Stormo

Article

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Insert

RPL1B

Use

Tags

mCherry

Expression

Yeast

Mutation

Removed introns, shuffled codons to vary nucleoti…

Promoter

S. cerevisiae GAL1

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNArodA

Plasmid

#149657

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAflaB

Plasmid

#149589

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.2

Plasmid

#149431

Purpose

miniTdg gene, SUMOylation mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

K341A SUMOylation mutant, SNPs of Tdg gene in OLA…

Promoter

Tdg promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-FOXA1

Plasmid

#139304

Purpose

Knocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1

Depositor

Claes Wadelius

Article

Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

Insert

shRNA and amiRNA against FOXA1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND4_L17_S24_S11_S19_S13

Plasmid

#132732

Purpose

T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND4_L17_S24_S11_S19_S13

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAG_Ptet*_ToeRep_N01_switch

Plasmid

#132737

Purpose

Inducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_ToeRep_N01_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_Ptet*_3WJrep_N19_switch

Plasmid

#132739

Purpose

Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_3WJrep_N19_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND3_L11_S11_S13_S19

Plasmid

#132731

Purpose

T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND3_L11_S11_S13_S19

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1-ATG7 3'UTR(406)

Plasmid

#122070

Purpose

Expressing ATG1 with ATG7 3'UTR

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

The native 3'UTR was replaced by ATG7 3'…

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-CD5cmyc2-bglpA

Plasmid

#110645

Purpose

Expression plasmid for c-Myc-tag embedded into human CD52 with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CDcmyc2-bglpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4A RNAiR

Plasmid

#115172

Purpose

expresses RNAi-resistant Sema4A with N-terminal myc tag

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

Sema4A (Sema4a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Silent mutation between amino acids in ORF (401-4…

Promoter

CMV

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC84-ScSEIPIN-Ndelta

Plasmid

#96985

Purpose

Express yeast SEIPIN gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

ScSEIPIN (SEI1 Budding Yeast)

Use

Tags

6xHis and GFP

Expression

Plant

Mutation

N-terminal 39 nucleotides were deleted from yeast…

Promoter

35S

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRS315-PGK-SEIPIN2

Plasmid

#96987

Purpose

Express GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN2 (AT1G29760 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

Phosphoglycerate Kinase (PGK) promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC84-SEIPIN3N-1C

Plasmid

#96983

Purpose

Express mutated Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Use

Tags

6xHis and GFP

Expression

Plant

Mutation

A segment of 507 nucleotides from the N terminus…

Promoter

35S

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC84-SEIPIN1N-3C

Plasmid

#96982

Purpose

Express mutated Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Use

Tags

6xHis and GFP

Expression

Plant

Mutation

A segment of 108 nucleotides from the N terminus…

Promoter

35S

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Use

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

Plasmid

#91933

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc

Use

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJK287

Plasmid

#71120

Purpose

Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coli

Depositor

T. J. Kappock

Article

Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

Insert

formyl-CoA:oxalate CoA-transferase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-mTmod3-150-352_domain

Plasmid

#70755

Purpose

mouse Tropomodulin3, 150-352 domain for bacterial expression

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 150-352 AA fragment (Tmod3 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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