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Plasmid#111457PurposeTransient expression of Flag-tagged human Sox9 with T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A/T240A
Plasmid#111458PurposeTransient expression of Flag-tagged human Sox9 with T236A/T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT236A and T240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mGSDMD1-251
Plasmid#111565PurposeExpress ELANE-cleaved mouse GSDMD in mammalian cellsDepositorInsertGasdermin D (Gsdmd Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1-NF2
Plasmid#166473PurposeExpresses fusion of mEGFP and NF2DepositorInsertmEGFP, NF2 (NF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
B4GALT1-Dox
Plasmid#124633PurposeDoxycycline-inducible B4GALT1 expression in mammalian cellsDepositorInsertsBeta-(1,4)-Galactosyltransferase 1
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1 YAPS127A- L325E
Plasmid#166464PurposeExpresses fusion of mEGFP and YAPS127A- L325EDepositorInsertmEGFP, YAPS127A- L325E (YAP1 Human)
UseTagsExpressionMammalianMutationYAPS127A- L325EPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m
Plasmid#161522PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editingDepositorInsertTadA(wt)-TadA(7.10)-zSpCas9(D10A)
UseCRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRPyCAG-fmKlf4-DsRed-Ip
Plasmid#26274DepositorInsertKlf4 (Klf4 Mouse)
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C72A
Plasmid#122477Purposeexpresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …PromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PatoM-LOG241
Plasmid#72847PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 backbone.DepositorInsertPatoM
UseTagsExpressionBacterialMutationPromoterPatoMAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmiR-96 cluster promoter
Plasmid#62517PurposemicroRNA 183-96-182 cluster promoter luciferase plasmidDepositorInsertpromoter of microRNA-183-96-182 cluster
UseTagsRenSP (optimized renilla luciferase gene)ExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CNC3G (CASANOVA-C3 with glycine linkers)
Plasmid#137192PurposeExpresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory proteinDepositorInsertCNC3G (CASANOVA-C3 with glycine linkers)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1 YAPS127A- L318E
Plasmid#166465PurposeExpresses fusion of mEGFP and YAPS127A- L318EDepositorInsertmEGFP, YAPS127A- L318E (YAP1 Human)
UseTagsExpressionMammalianMutationYAPS127A- L318EPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
IFITM3-N21del-V93-TAG
Plasmid#122480Purposeexpresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsExpressionMammalianMutationChanged Valine 93 to amber codon and deleted amin…PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
NG1301 pCI-TPI-4MS2-SMG5(short)-4H
Plasmid#65806PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4 MS2 stem loops, SMG5 3'UTR shortened versi…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C105A
Plasmid#122478Purposeexpresses human IFITM3-C105A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …PromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-V93-TAG
Plasmid#122479Purposeexpresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Valine 93 to amber codonPromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C71A
Plasmid#122476Purposeexpresses human IFITM3-C71A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …PromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad3
Plasmid#89828PurposeKnockdown human Smad3 expression in human mammalian cellsDepositorInsertSMAD3 shRNA (SMAD3 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only