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Showing: 2681 - 2700 of 4606 results

  8. RABEP1A-c019

    Plasmid
    #175477
    Purpose
    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABEP1 (RABEP1 Human)
    Use
    Tags
    TEV-TwinStrep
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175477-plasmid-map-sequence-id-353250

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  9. RABGEF1A-c014

    Plasmid
    #175478
    Purpose
    Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABGEF1 (RABGEF1 Human)
    Use
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175478-plasmid-map-sequence-id-353251

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  10. SYKA-c014

    Plasmid
    #175488
    Purpose
    Protein expression in insect cells. Entire CDS, M1-N635. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    SYK (SYK Human)
    Use
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175488-plasmid-map-sequence-id-353252

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  11. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    Use
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

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  12. pDONR223 SARS-CoV-2 NSP12_nostop

    Plasmid
    #149314
    Purpose
    Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    149314-plasmid-map-sequence-id-296449

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  13. pDONR223 SARS-CoV-2 NSP13_nostop

    Plasmid
    #149315
    Purpose
    Gateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    149315-plasmid-map-sequence-id-296463

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  14. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

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  17. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-131866

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  18. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

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  20. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    70703-plasmid-map-sequence-id-127765

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Showing: 2681 - 2700 of 4606 results