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Showing: 2681 - 2700 of 3066 results

  1. 1993_pCCLsin.PPT.hPGK.KaedeCETN1.PRE

    Plasmid
    #231804
    Purpose
    Label centrosomes with lentivirus by encoding a fusion protein consisting of the photo-convertible protein Kaede fused to Centrin 1 (CETN1), an integral component of centrosomes.
    Depositor
    Annette Oxenius
    Insert
    Kaede-CETN1 (Cetn1 Mouse)
    Use
    Lentiviral
    Tags
    Kaede
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231804-plasmid-map-sequence-id-460688

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  2. polySIM

    Plasmid
    #221068
    Purpose
    Expression of polySIM protein under T7 promoter
    Depositor
    Michael Rosen
    Article
    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33
    Insert
    10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4
    Use
    Tags
    His and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    unknown
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    221068-plasmid-map-sequence-id-459203

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  4. Myc-Nix-S35D

    Plasmid
    #197563
    Purpose
    Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-35. Based on Myc-Nix (#100795)
    Depositor
    Joseph Gordon
    Insert
    BCL2 interacting protein 3 like (BNIP3L Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Serine 35 changed to aspartic acid
    Promoter
    CMV
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197563-plasmid-map-sequence-id-386250

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  5. Venus-Bik-L61G-pEGFP-C1

    Plasmid
    #177426
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Leucine 61 replaced with glycine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177426-plasmid-map-sequence-id-348449

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  6. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid
    #177427
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycines 153 and 154 mutated to alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177427-plasmid-map-sequence-id-348452

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  7. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid
    #177428
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycine 154 replaced with STOP codon
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177428-plasmid-map-sequence-id-348454

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  13. GMIP

    Plasmid
    #32046
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GMIP (GMIP Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 80-357 only
    Promoter
    Available since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pJT1

    Plasmid
    #98299
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    98299-plasmid-map-sequence-id-189966

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  19. pV1F

    Plasmid
    #205084
    Purpose
    Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.
    Depositor
    Suomeng Dong
    Article
    Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.
    Insert
    AtVIP1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    agorbacterium virB operon promoter
    Available since
    Aug. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    205084-plasmid-map-sequence-id-405743

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Showing: 2681 - 2700 of 3066 results