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Plasmid#102909PurposePiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.DepositorUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAG497 mito-iFAST
Plasmid#130819PurposeExpresses mito-iFAST in mammalian cells (mitochondria labeling)DepositorInsertmito-iFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-HisFlag-rUSP19ER-CRmut
Plasmid#155253Purposefor mammalian overexpression of the mutated ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) CR mutant (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationCys(545) to ArgPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER deltaN3
Plasmid#155243Purposefor mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19DepositorInsertUSP19 (delta1-1631) (ER isoform) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER deltaN2
Plasmid#155242Purposefor mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19DepositorInsertUSP19 (delta1-1088) (ER isoform) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER deltaN1
Plasmid#155241Purposefor mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19DepositorInsertUSP19 (delta1-536) (ER isoform) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pfmh-hCry1
Plasmid#25843DepositorInserthuman Cryptochrome 1 (CRY1 Human)
UseTagsflag, his, and mycExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
ELL3 Streptag
Plasmid#127282PurposeExpresses mouse ELL3 with streptactin tag; does it degrade Myc?DepositorInsertELL3 (NM_145973) (ELL3 Mouse)
UseTagsTwin strep-tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCMV6M-Pak1 P191G, P192A
Plasmid#12213DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationPIX (PAK-interacting exchange factor) binding mut…PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG471 Lyn11-iFAST
Plasmid#130817PurposeExpresses Lyn11-iFAST in mammalian cells (inner plasma membrane labeling)DepositorInsertLyn11-iFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH340-SUP45(1-242)-GalBD
Plasmid#29740DepositorInsertSUP45 (SUP45 Budding Yeast)
UseTagsGal Activation domain and MycExpressionYeastMutationCodons 1-242 of the yeast SUP45 gene onlyPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
Golgi-FAST-IRES-iRFP670
Plasmid#216345PurposeFor testing localization of pFAST and iRFP670 proteins to Golgi complexDepositorInsertST6Gal1_pFAST-IRES-Sys1_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Peroxisomes-FAST-IRES-iRFP670
Plasmid#216347PurposeFor testing localization of pFAST and iRFP670 proteins to peroxisomesDepositorInsertpFAST_SKL-IRES-iRFP670_PXMP2
UseTagsmyc for FAST protein onlyExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-FAST-IRES-iRFP670
Plasmid#216346PurposeFor testing localization of pFAST and iRFP670 proteins to mitochondriaDepositorInsertCox8_pFAST-IRES-Tomm20_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-FAST-IRES-iRFP670
Plasmid#216344PurposeFor testing localization of pFAST and iRFP670 proteins to endoplasmic reticulumDepositorInsertPRL_pFAST-IRES-CANX_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only