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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

Plasmid

#132682

Purpose

AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

AAV

Tags

6xHIS and Flag

Expression

Mutation

Promoter

Chicken b-actin

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad1 (4SP/AP+AAVA)

Plasmid

#14955

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (4SP/AP+AAVA) (SMAD1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Four Serine to Alanine mutations in the linker re…

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172504

Purpose

Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1-N100 S449A/T450A/S454A

Plasmid

#19849

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1-N100 S449A/T450A/S454A (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

deleted amino acids 1-100 and changed Serine 449,…

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pGSTag Flag Jnk1a1

Plasmid

#15743

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1a1 (MAPK8 Human)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pLaNGo11-1

Plasmid

#126053

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11

Use

Binary

Tags

GFP1-10

Expression

Plant

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1b2

Plasmid

#13752

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b2 (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
BDS-2 3x WT (p53 binding site)

Plasmid

#16515

Depositor

Bert Vogelstein

Article

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

p53 binding site (SFN Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only
NELFE-mOrange pHis-//

Plasmid

#53303

Purpose

Expresses His-tagged NELF-E-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

NELF-E protein (Nelf-E Fly)

Use

Tags

His and mOrange

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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FL-fibulin-3 pcDNA4

Plasmid

#29700

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
GST-BRD (1079-1198)

Plasmid

#99507

Purpose

Bacterial expression of mouse CBP bromodomain

Depositor

Peter Wright

Article

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

mouse CBP BRD (1079-1198) (Crebbp Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pconst #1-RFP

Plasmid

#81075

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter - BBa_J23119

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBstSK+IRES-V5-IPpoI (mutant)

Plasmid

#206322

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag-mutated IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein (mutant version)

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_D27NDHFR_WTTS

Plasmid

#91244

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

D27N

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_F31YG121VDHFR_WTTS

Plasmid

#91229

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

F31YG121V

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_G121VDHFR_WTTS

Plasmid

#91233

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

G121V

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2b2

Plasmid

#13757

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b2 (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-JNKAR1-NES

Plasmid

#84632

Purpose

Unimolecular, FRET-based JNK activity reporter; cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ECFP-FHA1-JNKsubDD-Citrine-NES

Use

Tags

6xHis, Citrine, ECFP, Nuclear export signal (NES)…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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deltaPYD-NLRP11.MYC

Plasmid

#131197

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

deltaPYD-NLRP11 (NLRP11 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

deletion of the pyrin domain (amino acids 1-124 m…

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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