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GFP-Rab27B

Plasmid

#89447

Purpose

Expression of GFP-tagged human Rab27B in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B (RAB27B Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Rab35 WT

Plasmid

#47424

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 (RAB35 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST/hCx43-EGFP-N1

Plasmid

#40907

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 (GJA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6-150

Plasmid

#91777

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

GENIE Project, Douglas Kim

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-150

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-PH-ARNO2G-I303Ex2

Plasmid

#116868

Purpose

PIP3 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

CYTH2 (CYTH2 Human)

Use

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amind acids 252-399, isoleucine 303 changed to gl…

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Integrin β4-GFP

Plasmid

#205092

Purpose

Express GFP-tagged human integrin β4 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 4 (ITGB4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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Kif5A-GFP-CIBN

Plasmid

#102252

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

Kif5A-GFP-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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CRY2-mCherry-Miro1TM

Plasmid

#102247

Purpose

Expresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

CRY2PHR-mCherry-Miro1TM (RHOT1, CRY2 Mustard Weed, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KA

Plasmid

#133582

Purpose

Expresses IP3KA GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

Human inositol 1,4,5-trisphosphate 3-kinase A GFP tagged (ITPKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only
pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

Plasmid

#170273

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

See Comments

Promoter

CMVd1

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-DeAct-SpvB

Plasmid

#89446

Purpose

Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

CMV

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM106B-EGFP

Plasmid

#210366

Purpose

Expresses TMEM106B fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TMEM106B (TMEM106B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-PI35P2

Plasmid

#92419

Purpose

Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

Depositor

Geert van den Bogaart

Article

Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

Insert

MCOLN1 (Mcoln1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Synthetic gene, codon optimized for human.

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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GLG1-EGFP

Plasmid

#210372

Purpose

Expresses GLG1 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

GLG1 (GLG1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rab35 S22N inactive

Plasmid

#47426

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 S22N (RAB35 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

S22N inactive

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-hATG7wt

Plasmid

#87867

Purpose

Express a wild type human Atg7/Apg7 in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

ATG7 (ATG7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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hRAD18 dSAP-EGFP

Plasmid

#68825

Purpose

Expresses human RAD18 (deleting SAP domain) tagged with EGFP

Depositor

Satoshi Tateishi

Article

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 (deleting SAP domain) tagged with EGFP (RAD18 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
hRAD18 dC2-EGFP

Plasmid

#68826

Purpose

Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

Depositor

Satoshi Tateishi

Article

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pphiLOV3-N1

Plasmid

#178973

Purpose

Expresses phiLOV3 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

phiLOV3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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PEX3*-SBP-GFP

Plasmid

#120174

Purpose

Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Peroxisomal biogenesis factor 3 (PEX3 Human)

Use

Tags

SBP and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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