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C122-E16: CyPet

Plasmid

#162896

Purpose

Standard Gateway entry clone encoding CyPet fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

CyPet fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKD2.RNAi

Plasmid

#10816

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKD2 RNAi (PRKD2 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pET28b-Sad1

Plasmid

#90297

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-KanMx

Plasmid

#122896

Purpose

ADE2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB2375

Plasmid

#67546

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-HphMx

Plasmid

#122895

Purpose

ADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-URA3

Plasmid

#122886

Purpose

ADE2-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C122-E17: mPlum

Plasmid

#162897

Purpose

Standard Gateway entry clone encoding mPlum fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Plum fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB2224

Plasmid

#67547

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE TRP1-KanMx

Plasmid

#122902

Purpose

TRP1-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE TRP1-NatMx

Plasmid

#122903

Purpose

TRP1-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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p153 pPr-cre

Plasmid

#8403

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

probasin driven Cre

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pAMB_CAT-FspI

Plasmid

#61002

Purpose

T7-transcription template for CAT control mRNA used for in vitro translation

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

Chloramphenicol acetyltransferase

Use

Unspecified

Tags

Expression

Mutation

FspI site introduced after T7 terminator

Promoter

T7

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-NatMx

Plasmid

#122897

Purpose

ADE2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8818

Plasmid

#126909

Purpose

Template for PCR amplification of HMG1from Yarrowia lipolytica

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

YlHMG1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-HIS3

Plasmid

#122887

Purpose

LYS2-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-MET17

Plasmid

#122889

Purpose

LYS2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE TRP1-LEU2

Plasmid

#122892

Purpose

TRP1-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE ADE2-LEU2

Plasmid

#122884

Purpose

ADE2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-sad1-C63A

Plasmid

#90302

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Use

Tags

His tag

Expression

Bacterial

Mutation

C63A

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge