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pLib6.4B-Aalb_726

Plasmid

#176675

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aaeg_763

Plasmid

#176672

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Aaeg_972

Plasmid

#176660

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Cquin_801

Plasmid

#176657

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Cquin_801

Plasmid

#176671

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Cquin_728

Plasmid

#176670

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet1-His-TEV-OTC_Ub

Plasmid

#172134

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265H

Plasmid

#149554

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

hAID-ziSpyMacCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ266B

Plasmid

#149555

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

ziSpyMacCas9(D10A)-PmCDA1-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ262m3

Plasmid

#149556

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHES 946

Plasmid

#112644

Purpose

blue light inducible cryptochrome based optogenetic expression system

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Synthetic, Budding Yeast, Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAFMW-AthHEN1-CM

Plasmid

#104981

Purpose

Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Use

Tags

FLAG Myc

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Banshee-ShCit-A

Plasmid

#85216

Purpose

Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

shCit-A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Banshee-ShCit-B

Plasmid

#85217

Purpose

Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

shCit-B

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTAct2 (GB0210)

Plasmid

#68191

Purpose

Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 terminator

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p5'UTRAct2 (GB0193)

Plasmid

#68178

Purpose

Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

5'UTR AtAct2

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-hRubicon

Plasmid

#64144

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

Rubicon (RUBCN Human)

Use

Tags

GAL4 activation domain

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pcdna3 EE Jip1b

Plasmid

#52127

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Use

Tags

EE

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-Flag-Tipin-L195A

Plasmid

#23118

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed Leucine 195 to Alanine

Promoter

Available since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
pcdna3 Flag Jip1b

Plasmid

#52123

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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