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psiCHECK2-CCNA2 LONG 3'UTR

Plasmid

#29472

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

CCNA2 LONG 3'UTR (CCNA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-LbCas12a-8xNLS

Plasmid

#209020

Purpose

Lentiviral vector expressing human codon-optimized LbCas12a with 8xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m

Insert

LbCas12a-8xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCSm5En2CER4(#457)

Plasmid

#184090

Purpose

cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-chkEn2(C>S)-ERT2

Use

Synthetic Biology

Tags

5xMyc

Expression

Mammalian

Mutation

chkEn2: C175S; ERT2: G400V, M543A, L544A, deleted…

Promoter

sCMV+SP6

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pT22gcfUbm5En25EE4PCh(#552)

Plasmid

#184092

Purpose

cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

5myc-chkEn2(5E)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22Ubm4Eng2bKRPC(#493)

Plasmid

#184091

Purpose

cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

4xMyc

Expression

Mutation

ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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W250R EPHA3 pcDNA3.1

Plasmid

#102744

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

W250R (TGG > AGG)

Promoter

CMV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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D678E EPHA3 pcDNA3.1

Plasmid

#102750

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

D678E (GAC > GAa)

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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T660K EPHA3 pcDNA3.1

Plasmid

#102749

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

T660K (ACA > AAA)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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S449F EPHA3 pcDNA3.1

Plasmid

#102748

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

S449F (TCC > TTC)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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A435S EPHA3 pcDNA3.1

Plasmid

#102747

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

A435S (GCT > TCT)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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N379K EPHA3 pcDNA3.1

Plasmid

#102745

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

N379K (AAT > AAG)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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T166N EPHA3 pcDNA3.1

Plasmid

#102742

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

T166N (ACT > AAT)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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D806N EPHA3 pcDNA3.1

Plasmid

#102754

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

D806N (GAT > aAT)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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K761N EPHA3 pcDNA3.1

Plasmid

#102752

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

K761N (AAG > AAt)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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R728L EPHA3 pcDNA3.1

Plasmid

#102751

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

R728 (CGA > CtA)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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T37K EPHA3 pcDNA3.1

Plasmid

#102740

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

T37K (ACA > AAA)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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N85S EPHA3 pcDNA3.1

Plasmid

#102741

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

N85S (AAC > AGC)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A-stop-mut

Plasmid

#53713

Purpose

Luciferase reporter assay for CIP2A with mutated firefly stop codon

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Tags

Expression

Mutation

Mutation on the stop codon of firefly luciferase …

Promoter

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
T7-VEE-OKS-iM

Plasmid

#58972

Purpose

RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Inserts

Oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
cMyc (MYC Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

26S subgenomic promoter and IRES

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pMaster12

Plasmid

#58527

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
Myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
NANOG (NANOG Human)
IRES
NR5A2 (NR5A2 Human)
miR302/367 (MIR367, MIR302A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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