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Plasmid#197373PurposeThe lentiviral vector for miRFP718nano(S86C) and EGFPDepositorInsertmiRFP718nano(S86C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationS86C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-iRFP713(V256C)-P2A-EGFP
Plasmid#198061PurposeThe lentiviral vector for iRFP713(V256C) and EGFPDepositorInsertiRFP713(V256C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationV256C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-6507-P2A-EGFP
Plasmid#197367PurposeThe lentiviral vector for NIR-GECO2(I426C) and EGFPDepositorInsertNIR-GECO2(I426C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationI426C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-GFP
Plasmid#28304DepositorInsertGFP
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMammalianMutationN/APromoterCAGAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
Syn-FRT-MCS-WPRE-SV40 - GCH9
Plasmid#230819PurposeAAV cloning vector for Flpo dependent expression under the Syn promoter.DepositorTypeEmpty backboneUseAAV; Cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-FRT-MCS-WPRE-SV40 - GCH21
Plasmid#230825PurposeAAV cloning vector for Flpo dependent expression under the CaMKII promoter.DepositorTypeEmpty backboneUseAAV; Cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FRT-MCS-WPRE-SV40 - GCH15
Plasmid#230822PurposeAAV cloning vector for Flpo dependent expression under the EF1a promoter.DepositorTypeEmpty backboneUseAAV; Cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFAP-FRT-MCS-WPRE-SV40 - GCH33
Plasmid#230831PurposeAAV cloning vector for Flpo dependent expression under the GFAP promoter.DepositorTypeEmpty backboneUseAAV; Cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-miRFP670nano(R57C/C86S)-P2A-EGFP
Plasmid#197370PurposeThe lentiviral vector for miRFP670nano(R57C/C86S) and EGFPDepositorInsertmiRFP670nano(R57C/C86S)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationR57C/C86S mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-iRFP713(C15S/V256C)-P2A-EGFP
Plasmid#198062PurposeThe lentiviral vector for iRFP713(C15S/V256C) and EGFPDepositorInsertiRFP713(C15S/V256C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationC15S/V256C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-6504-P2A-EGFP
Plasmid#197368PurposeThe lentiviral vector for NIR-GECOHI and EGFPDepositorInsertNIR-GECO2(C18S/I426C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationC18S/I426C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-mCherry-DMDEx23-eGFP
Plasmid#211366PurposePiggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)DepositorInsertmCherry-DMDEx23-eGFP
UseTagsExpressionMammalianMutationmCherry interrupted by mdx dystrophin exon 23 bet…PromoterCAGAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
miCBE
Plasmid#205413PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
UseTagsExpressionMammalianMutationD61A+E85R+H369R+S387R+S457RPromoterhU6, CAG, CMVAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
UseTagsGal8 is fused to the c-terminus of GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SpCas9D10A nickase
Plasmid#216737PurposeExpresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRTagsExpressionMammalianMutationD10APromoterCMVd1Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10878
Plasmid#183964PurposeCAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD156R, E292R, D235R, D350RPromoterCAGAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10873
Plasmid#183961PurposeU6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPRDepositorInsertsHyperdCas12a
poly-crRNA array targeting Sox2-Klf4-Oct4
UseCRISPRTagsHAExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAG and U6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pbG-APRT-J-u32uni-PdTKmCh
Plasmid#107279PurposeAPRT c.407T>C (APRT*J) donor vector with unilateral microhomologyDepositorInsertsUseTagsExpressionMutationc.402A>T, c.407T>CPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV(gRNA)-CMV-eGFP-U6(sgCTG)
Plasmid#216732PurposeContains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytesDepositorArticleInsertsgCTG lentiviral guide RNA with eGFP tag
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgCTG-CMV-GFP
Plasmid#216734PurposeAAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.DepositorArticleInsertsgCTG
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6 and CMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only