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Plasmid#138478PurposeExpresses HIV Tat for efficient sgRNA packaging.DepositorInsertTat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1
Plasmid#175766PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1DepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV10-hFIP200
Plasmid#24300DepositorInsertFIP200 (RB1CC1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2
Plasmid#192440PurposeEncodes mCherry-tagged wild-type TSC2 isoform 5.DepositorInsertmCherry-TSC2 (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLT4
Plasmid#116745PurposeLentiviral expression of FLT4DepositorInsertFLT4 (FLT4 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT18mobsacB
Plasmid#72648Purposesucrose counter-selection allelic exchange vector for Pseudomonas syringaeDepositorTypeEmpty backboneUseSucrose counter-selection allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGTVL2-CRBN-TBD
Plasmid#196369Purposeprotein expression of the GST-tagged Cereblon Thalidomide binding domainDepositorInsertCRBN (CRBN Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationcontains only T319-T425PromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-eGFP-MEFV
Plasmid#92103PurposeExpression in eukaryotic cells and generating stable cell linesDepositorInsertMEFV (MEFV Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-REVERBA-mScarlet-NLS-PEST
Plasmid#190658PurposeFluorescent reporter for circadian rhythmsDepositorInsertReverba-mScarlet (Nr1d1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP1-10_pcDNA3.1(-)
Plasmid#182931PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragmentDepositorTypeEmpty backboneUseTags3C-cleavable split GFP 1-10, FLAG tag, and HA sig…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
RnBarr2-mCherry
Plasmid#137794PurposeVisualization of beta-arrestin2DepositorInsertRat beta-arrestin2 (Arrb2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-13 ONE-GO
Plasmid#189740PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationL342M (present in YFP but no functional consequen…PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX GFP T2A Cre
Plasmid#63704PurposeInducible expression of GFP T2A Cre fusion in mammalian cellsDepositorInsertT2A Cre
UseRetroviralTagsEGFPExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSAP
Plasmid#206429PurposeA vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA-EGFP
Plasmid#39862DepositorInsertmonomeric streptavidin fused to EGFP
UseTags6xHis, EGFP, and FLAGExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 TFAM-mScarlet
Plasmid#129573PurposeExpression of mScarlet fused TFAMDepositorInsertTFAM (TFAM Human)
UseTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
psPax2-D64V-NC-mNeon
Plasmid#196509PurposemNeon fluorescent protein fused to nucleocapsid protein in psPAX2DepositorInsertmNeon
UseTagsmNeonExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R
Plasmid#58978Purposeexpresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
UseTagsExpressionBacterialMutationPromoterT7/Sp6Available sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax
Plasmid#222138PurposeAdenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9 (cas9 Synthetic)
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spRYCas9-ABE-UMax
Plasmid#222139PurposeNear PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertTadA-nSpRYCas9
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pbabe-c-mycT58A+HRasG12V
Plasmid#11130DepositorUseRetroviralTagsExpressionMammalianMutationc-myc T58A, A156T, and G438R; and H-Ras G12VPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSm-Nuclease
Plasmid#132431PurposeExpresses Serratia marcescens nuclease in E. coli using T7 promoterDepositorInsertSerratia marcescens nuclease (codon optimized)
UseTagsNoneExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-lck-jGCaMP8f
Plasmid#176759PurposeAAV-mediated expression of membrane bound GCaMP8f under the control of GfaABC1D promoterDepositorHas ServiceAAV1 and AAV9Insertlck-jGCaMP8f
UseAAVTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIN-TRE-H2BeGFP-rtTA2
Plasmid#165494PurposeDoxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.DepositorInsertHuman histone H2B (H2BC21 Human)
UseLentiviralTagsEGFPExpressionMutation-PromoterTetO-CMVAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE Hygro Flag hTERT
Plasmid#53546Purposeretroviral expression of human TERTDepositorInsertTERT (TERT Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-SZT2
Plasmid#87034Purposemammalian expression of SZT2DepositorInsertSZT2 (SZT2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pChlamy/Cre
Plasmid#205440PurposeAn expression vector for Cre recombinase (codon usage for C. reinhardtii)DepositorInsertCre recombinase gene
UseChlamydomonas expressionTagsExpressionMutationPromoterHeat shock protein 70A (HSP70A)/ribulose bisphosp…Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
iF53 PB-Zim3-dCas9-mScarlet-Hygro
Plasmid#204726PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
AbVec1.1-IGLC2-XhoI
Plasmid#99575PurposeExpression of immunoglobulin light chains in mammalian cells, human lambda 2 constant regionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0576
Plasmid#143886PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXA10 (HOXA10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti_gRNA-Puro
Plasmid#84752PurposeExpresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS133
Plasmid#211504PurposeAAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertmTagBF2
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMF1762
Plasmid#125583PurposeExpresses mitochondrial roGFP2 (mt-roGFP2) in mammalian cellsDepositorInsertmt-roGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-DEST-MatryoshCaMP6s
Plasmid#100025PurposeMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTagsV5 Epitope TagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCD8-GFP in pUAST
Plasmid#17746DepositorInsertmouse CD8 (Cd8a Mouse)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSET his-mCherry
Plasmid#113552PurposeFor bacterial expression and purification of mCherryDepositorInserthis-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-RIPK2
Plasmid#23846DepositorInsertRIPK2 (RIPK2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC10A1_STOP
Plasmid#161073PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC10A1 (SLC10A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 proHB-EGF CS
Plasmid#11601DepositorInsertHB-EGF (HBEGF Human)
UseTagsHis and mycExpressionMammalianMutationConstitutively secreted HB-EGF: C-terminal amino …PromoterAvailable sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET3a-OSF-CypA
Plasmid#79039PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertOneSTrEP-FLAG- Cyclophilin A (PPIA Human)
UseTagsOneSTrEP-FLAGExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo-CnA
Plasmid#191966PurposeExpression of CnA (calcineurin subunit alpha)DepositorInsertCalcineurin subunit alpha (PPP3CA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)
Plasmid#214885PurposeLentiviral vector encoding TMP-regulatable RfxCas13d-DD fusionDepositorInsertEF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-EYFP
Plasmid#38011DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsEYFP and SNAP26mExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-AOX1-eGFP
Plasmid#126718Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS
Plasmid#127656PurposeOver-express NLS-FLAG-cGASDepositorInsertcGAS (CGAS Human)
UseLentiviralTagsFlagExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-ecfp
Plasmid#65030PurposeGmR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
ADORA2A-Tango
Plasmid#66210PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertADORA2A (ADORA2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBItetDT960GFP
Plasmid#80419Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 960 interrupted CTG repeats in exon 15 locatedDepositorInsertEGFP and human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ClopHensor
Plasmid#25938DepositorInsertClopHensor
UseTagsStrep-IIExpressionMammalianMutationE2GFP is EGFP-T203YPromoterAvailable sinceSept. 13, 2010AvailabilityAcademic Institutions and Nonprofits only