-
Plasmid#213220PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR3 (CXCR3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-FLTID-GSQ-PMLivaWT-P2A-BLAST
Plasmid#208040PurposeEnables constitutive expression of N-terminal TurboID-fused PML (isoform IVa; WT); selection with blasticidinDepositorInsertPML (isoform IVa) (PML Human)
UseLentiviralTagsFLAG, TurboIDExpressionMutationPromoterEFSAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C - AVITEV
Plasmid#74058Purposeretroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX Abl-SH2
Plasmid#46402DepositorInsertAbl (ABL1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 120-225PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF6_P2A_Hygro_Barcode
Plasmid#120492PurposeBarcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF6 (KLF6 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo 1-159
Plasmid#186016Purpose2xHis-LRRK2 Armadillo 1-159DepositorInsert2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)
UseTagsExpressionBacterialMutationSee depositor's comments below.PromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo 350-550
Plasmid#186018Purpose2xHis-LRRK2 Armadillo 350-550DepositorInsert2xHis-LRRK2 Armadillo 350-550 (LRRK2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pan-Neurofascin scFv [L11A/41]
Plasmid#206722PurposeMammalian Expression of Pan-Neurofascin scFV. Derived from hybridoma L11A/41 scFv.DepositorInsertPan-Neurofascin (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer
Plasmid#176589PurposeEndogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Chesapeake Bay) locus
UseTagsExpressionBacterialMutationTruncated CRISPR array to two direct repeats, rep…PromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-STK31
Plasmid#116796PurposeLentiviral expression of STK31DepositorInsertSTK31 (STK31 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
Neuroligin-2 scFv [L107/90]
Plasmid#206763PurposeMammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/90 scFv.DepositorInsertNeuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Parvalbumin scFv [L114/3]
Plasmid#182072PurposeMammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/3.DepositorInsertrecombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα1(I)
Plasmid#119844PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PRKD1
Plasmid#116778PurposeLentiviral expression of PRKD1DepositorInsertPRKD1 (PRKD1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-OXA1L
Plasmid#116768PurposeLentiviral expression of OXA1LDepositorInsertOXA1L (OXA1L Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _SH3bm
Plasmid#59141PurposeYAP1 mutants that harbored a deletion of SH3 binding motif, which disrupts interaction of YES1 with YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationSH3 domain deleted (aa278-290)PromoterAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc3-Mdm2 p60
Plasmid#52059Purposeexpression of truncated Mdm2 in mammalian cellsDepositorInsertMdm2 p60 (truncated) (MDM2 Human)
UseTagsmyc3ExpressionMammalianMutationp60 (truncated at D367, DVDP motif)PromoterCMVAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-E
Plasmid#154418PurposeGateway-compatible Entry vectorDepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of e…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX CblB-SH2
Plasmid#46413DepositorInsertCblB (CBLB Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 42-359PromoterTacAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR2
Plasmid#167001PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 2 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR1
Plasmid#167000PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 1 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-RAB10 K154A
Plasmid#130886PurposeExpresses human RAB10 K154A mutant with N-terminal GFP tagDepositorInsertRAB10 (RAB10 Human)
UseLentiviralTagsGFPExpressionMammalianMutationK154APromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-M
Plasmid#154419PurposeGateway-compatible Entry vectorDepositorInsertM (M SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of m…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
NCKX4 scFv [N414/25]
Plasmid#190553PurposeMammalian Expression of NCKX4 scFV. Derived from hybridoma N414/25.DepositorInsertNCKX4 (Homo sapiens) recombinant scFV (Slc24a4 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)13
Plasmid#127526PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast POLD1
Plasmid#160805PurposeExpress POLD1DepositorInsertPOLD1 (POLD1 Human)
UseRetroviralTagsExpressionMutationCodon optimized C-terminal 230 amino acidsPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1605-shRNAres_W
Plasmid#147889PurposeMammalian Expression of HsNot1iso1_1-1605-shRNAresDepositorInsertHsNot1iso1_1-1605-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1093-1317_W
Plasmid#147892PurposeMammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests).DepositorInsertHsNot1iso1_1093-1317 (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1318-1605_W
Plasmid#147893PurposeMammalian Expression of HsNot1iso1_1318-1605DepositorInsertHsNot1iso1_1318-1605 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1840-shRNAres_W
Plasmid#147891PurposeMammalian Expression of HsNot1iso1_1-1840-shRNAresDepositorInsertHsNot1iso1_1-1840-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pGEX Btk-SH2
Plasmid#46412DepositorInsertBtk (BTK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 271-385PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC Ser62Ala_P2A_Hygro_Barcode
Plasmid#120514PurposeBarcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Ser62Ala (MYC Human)
UseLentiviralTagsExpressionMutationPoint mutation changing Serine to Alanine at amin…PromoterEF1aAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75236PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR3
Plasmid#167002PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 3 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-K292A
Plasmid#134575PurposeExpress human DDX43 mutant (K292A, ATPase dead) in E. coliDepositorInsertDDX43-K292A (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (K292A)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)
Plasmid#119840PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mApple between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-hAGT-His
Plasmid#167276PurposeOverexpression of the human o_-alkylguanine-dna alkyltransferase in E. coliDepositorInserthAGT (AGT Human)
UseTagsHis x10 and Strep-tagExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only