-
Plasmid#105447PurposeCation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChrimsonR-mRuby2-ST
UseTagsmRuby2 and soma targeting motifExpressionMammalianMutationK176R in ChrimsonPromoterpCAGGSAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
mMaroon-PAK-mMaroon
Plasmid#84361PurposeFRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroonDepositorInsertmMaroon-PAK-mMaroon
UseTagsmMaroon flanking PAK domain on N and C terminusExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_HUWE1_3xFLAG
Plasmid#187155Purposemammalian cell expression of HUWE1-3xFLAGDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-TVA-IRES-Puro
Plasmid#172366PurposeGeneration of TVA-expressing stable cell lines, resistant to the antibiotic PuromycinDepositorInsertTVA + Puro
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDsRed2-Keap1
Plasmid#21551DepositorInsertkelch-like ECH-associated protein 1 (KEAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE C-terminal
Plasmid#137178PurposeAAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOS018: ratiometric pHluorin (mitchondrial)
Plasmid#163053Purposeratiometric pHluorin (mitchondrial)DepositorInsertratiometric pHluorin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP680-N1
Plasmid#136557PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)DepositorInsertmiRFP680
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Phusion
Plasmid#206210PurposeInducible expression of DNA polymeraseDepositorInsertPhusion DNA polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-Turbo-YFP-NLS
Plasmid#127368PurposeBinary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
UseTagsGS linker, NLS, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1âŠPromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT-T7-KPNA2
Plasmid#26678DepositorInsertKaryopherin Alpha 2 (KPNA2 Human)
UseTagsT7 (MASMTGGQQMG)ExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMOS023: Peredox NADH/NAD+ sensor (cytosolic)
Plasmid#163060PurposePeredox NADH/NAD+ sensor (cytosolic)DepositorInsertPeredox
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGEâŠPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro-ARID1A
Plasmid#39478PurposeTetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vectorDepositorInsertARID1A (ARID1A Human)
UseLentiviralTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-pBAD
Plasmid#108315PurposeGFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.DepositorInsertGFP under control of arabinose-inducible promoter pBAD
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV14-3X-Flag-SARS-CoV-2 S
Plasmid#145780PurposeOver expression of SARS-CoV-2 S (spike) in mammalian cell lineDepositorInsertSARS-CoV-2 S (S SARS CoV 2)
UseTags3xFlagExpressionMammalianMutationCodon optimized and C-terminal 19 amino acids delâŠPromoterCMVAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
hCD4-mOrange
Plasmid#110192PurposeEncodes for human CD4 coding sequence tagged with the mOrange FPDepositorInserthCD4 (CD4 Human)
UseTagsmOrangeExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
LAMP-ReNL_pcDNA3
Plasmid#89532PurposeRed color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1DepositorInsertReNL
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNAJB1_DNAJ
Plasmid#109918PurposeProtein expression and purification of DNAJB1_DNAJDepositorInsertDNAJB1_DNAJ
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
UbcH5c WT pET28a
Plasmid#12643DepositorInsertUbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-hAos1
Plasmid#53135PurposeE.coli expression of full length human Aos1 (SAE1) with Nterminal His-tagDepositorInsertAos1 (SAE1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Green Glifon4000
Plasmid#126208Purposegreen fluorescent glucose sensor for mammalian cellsDepositorInsertGreen Glifon4000
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3e UbB polyA
Plasmid#188702PurposeGateway 3' element encoding the ubiquitin B polyadenylation sequenceDepositorInsertUbB polyA (ubb Zebrafish)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
TA1-Tango
Plasmid#66508PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertTA1 (TAAR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-GCaMP5G
Plasmid#105009Purposemitochondrial calcium sensorDepositorInsertGCaMP5G
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa
Plasmid#127389PurposeFluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alphaDepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationPromoterpCAGAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRecT_2
Plasmid#167546PurposeEncodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.DepositorInsertE. faecium Com15 recT under an IPTG inducible promoter, with lacI
UseTagsExpressionBacterialMutationPromoterIPTGAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mCherry-C
Plasmid#203326PurposeExpress N-terminal mCherry-fused protein in mammalian cellDepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-YFP
Plasmid#1816PurposeMammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
UseTagsYFPExpressionMammalianMutationD50E (not important for function of plasmid)PromoterAvailable sinceFeb. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-TOP-dGFP-reporter
Plasmid#14715PurposeBeta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).DepositorInsertLEF-1/TCF-responsive promoter (Ctnnb1 )
UseLentiviralTagsd2 eGFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAF112
Plasmid#135920PurposeAll-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.DepositorTypeEmpty backboneUseLuciferase; TransposonTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-10A1
Plasmid#15805DepositorInsert10A1 Murine leukemia virus envelope mRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
mScarletI-Rab7
Plasmid#112955PurposeIn vivo visualization of Rab7 (can be used for colocalization studies)DepositorArticleInsertRab7
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-MITF-A
Plasmid#38132DepositorInsertMITF-A (MITF Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV- ÎșLight1.3
Plasmid#201223PurposeExpress kLight1.3 biosensor in mammalian cellsDepositorInsertÎșLight1.3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-KDR
Plasmid#23925DepositorInsertKDR (KDR Human)
UseGateway donor vectorTagsExpressionMutationV297IPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBT375
Plasmid#125616PurposeExpresses evoAPOBEC1-BE4max-NG in mammalian cellsDepositorInsertevoAPOBEC1-BE4max-NG
UseTagsExpressionMammalianMutationE4K H109N H122L D124N R154H A165S P201S F205SPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF2-ABCG2-IRES-Neo
Plasmid#25983DepositorInsertATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
UseLentiviralTagsEF1-apha promoter and IRES-NeoExpressionMammalianMutationNonePromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-mCherry-hPMCA2w/b
Plasmid#111568PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorUseAAVTagsmCherryExpressionMutationPromoterAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only