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Addgene
Showing: 2661 - 2680 of 16003 results

  1. pVITRO-Fab_Theo-LUCID

    Plasmid
    #119048
    Purpose
    Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Theo-LUCID_part1
    Fab_Theo-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Availability
    Academic Institutions and Nonprofits only
    119048-plasmid-map-sequence-id-231811

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  2. pVITRO-Fab_Quin-LUCID

    Plasmid
    #119049
    Purpose
    Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Quin-LUCID_part1
    Fab_Quin-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Availability
    Academic Institutions and Nonprofits only
    119049-plasmid-map-sequence-id-231810

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  4. pTHRB-donor

    Plasmid
    #112333
    Purpose
    CRISPR donor plasmid to tag human transcription factor THRB with GFP
    Depositor
    Kevin White
    Insert
    THRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112333-plasmid-map-sequence-id-228563

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  6. pGS1034

    Plasmid
    #63796
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1
    Insert
    CBP-3xFLAG-TEV-Protein A followed by KlLEU2
    Use
    E. coli cloning vector
    Tags
    CBP-3xFLAG-TEV-Protein A
    Expression
    Mutation
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
    63796-plasmid-map-sequence-id-110311

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  7. Yes-EGFP

    Plasmid
    #110497
    Purpose
    Fluorescent Yes
    Depositor
    Bernardo Mainou
    Insert
    Yes (YES1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    110497-plasmid-map-sequence-id-210495

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  8. EGFP-Rab13-Q67L

    Plasmid
    #110502
    Purpose
    Constitutive Active
    Depositor
    Bernardo Mainou
    Insert
    Rab13-Q67L (RAB13 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Q67L
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    110502-plasmid-map-sequence-id-210501

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  9. pSMPUW_6.5_M2_Ef1a-V5-gateway

    Plasmid
    #128441
    Purpose
    lentiviral destination vector with Ef1a promoter and N-terminal V5 tag
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter and N-terminal V5 tag
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    128441-plasmid-map-sequence-id-256457

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  10. pSMPUW_7.1_M2_hcSyn-gateway-V5

    Plasmid
    #128457
    Purpose
    lentiviral destination vector with hcSyn promoter and C-terminal V5 tag
    Depositor
    Lindy Barrett
    Insert
    hcSyn promoter and C-terminal V5 tag
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    128457-plasmid-map-sequence-id-270524

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  13. pET28a MDHC_YEAST

    Plasmid
    #204232
    Purpose
    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHC_YEAST (MDH2 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Availability
    Academic Institutions and Nonprofits only
    204232-plasmid-map-sequence-id-413337

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  14. pANY8

    Plasmid
    #164900
    Purpose
    Containing His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-ELP-Intein-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    164900-plasmid-map-sequence-id-330483

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Showing: 2661 - 2680 of 16003 results