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Showing: 2641 - 2660 of 2808 results

  2. pSL3927 (pDonor(plasmid)_Pse)

    Plasmid
    #200905
    Purpose
    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Insert
    Mini Tn7016 (pTarget NGS)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200905-plasmid-map-sequence-id-393162

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  5. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid
    #130640
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6
    Use
    CRISPR; Transposon
    Tags
    His10-MBP-TEVsite on TniQ
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130640-plasmid-map-sequence-id-255648

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  7. MGAT1-GFP

    Plasmid
    #163647
    Purpose
    Expresses GFP tagged MGAT1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    alpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163647-plasmid-map-sequence-id-322673

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  8. pSL0284 (pQCascade_entry)

    Plasmid
    #130635
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130635-plasmid-map-sequence-id-255022

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  9. pORTMAGE-1

    Plasmid
    #72680
    Purpose
    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    lower strength RBS before mutL E32K allele than i…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pSL0762 (pQCascade_crRNA-nt)

    Plasmid
    #130636
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130636-plasmid-map-sequence-id-255017

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  13. pSL0828 (pQCascade_crRNA-4)

    Plasmid
    #130637
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130637-plasmid-map-sequence-id-255021

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  14. pHM123DEnv

    Plasmid
    #137668
    Purpose
    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)
    Depositor
    Anjali Joshi
    Article
    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.
    Insert
    HIV-1 Envelope Glycoprotein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    137668-plasmid-map-sequence-id-273365

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  15. pAcGFP1-N1-SialT

    Plasmid
    #87324
    Purpose
    To express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    ST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
    Use
    Tags
    AcGFP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87324-plasmid-map-sequence-id-177636

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  17. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

    Plasmid
    #130639
    Purpose
    Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCas8, VchCas7, VchCas6
    Use
    CRISPR; Transposon
    Tags
    His10-MBP-TEVsite on Cas8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130639-plasmid-map-sequence-id-255644

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  18. cfmTurquoise-N

    Plasmid
    #139284
    Purpose
    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.
    Depositor
    Ikuo Wada
    Article
    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d
    Insert
    cfmTurquoise
    Use
    Tags
    Multi-cloning sites
    Expression
    Mammalian
    Mutation
    Cys48Ser and Cys70/Met
    Promoter
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    139284-plasmid-map-sequence-id-291944

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  19. pHM024Env

    Plasmid
    #137691
    Purpose
    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)
    Depositor
    Anjali Joshi
    Article
    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.
    Insert
    HIV-1 Envelope Glycoprotein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    137691-plasmid-map-sequence-id-273387

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  20. pSL0747 (pDonor_L-MmeI)

    Plasmid
    #130647
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (L*)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    TGTTGATG --> TGTTGGAG
    Promoter
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130647-plasmid-map-sequence-id-255647

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Showing: 2641 - 2660 of 2808 results