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Plasmid#193653PurposeExpression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLb-enCas12a-TadA*8e-V106W, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-D156R/G532R/K538R, TadA*8e-V106WPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHSN6I01
Plasmid#50587PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsdCas9-KRAB
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSExpressionMutationdCas9 that is defective in DNA cleavage; the maiz…Promoter2×35Sp and AtU6-26pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA
Plasmid#190790Purpose[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.DepositorInsertsCas9
mazF-LVA
UseCRISPR and Synthetic BiologyTagsLVAExpressionBacterialMutationsilent mutations of BpiI-sites in original Cas9 g…PromoterPnrsB and Ptrc-[E*](104)Available sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S2-cAMPr
Plasmid#160723PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.DepositorInsertS2-cAMPr
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF Con/Foff hChR2(H134R)-EYFP
Plasmid#55647PurposeCre-on/Flp-off ChR2-EYFP under the short Ef1a promoterDepositorInsertChR2(H134R)-EYFP
UseAAV; Cre on/flp off chr2-eyfpTagsEYFPExpressionMammalianMutationPromoterShort Ef1aAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hnEF Coff/Fon hChR2(H134R)-EYFP
Plasmid#55649PurposeCre-off/Flp-on ChR2-EYFP under the short Ef1a promoterDepositorInsertChR2(H134R)-EYFP
UseAAV; Cre off/flp on chr2-eyfpTagsEYFPExpressionMammalianMutationPromoterShort Ef1Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-GCaMP6f
Plasmid#160722PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-GCaMP6f
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBUN6A11
Plasmid#50579PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInsertsdCas9-VP64
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSExpressionMutationdCas9 = defective in DNA cleavage; 4×minimal VP16…PromoterOsU3p and Ubi1pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-enCas12a-A3A-Y130F
Plasmid#193652PurposeExpression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLb-enCas12a-hA3A-Y130F, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130FPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBUN6I11
Plasmid#50580PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInsertsdCas9-KRAB
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSExpressionMutationdCas9 that is defective in DNA cleavage; the maiz…PromoterOsU3p and Ubi1pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-ExRaiAKAR
Plasmid#160724PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.DepositorInsertS3-ExRaiAKAR
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S4-ExRaiCKAR
Plasmid#160725PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.DepositorInsertS4-ExRaiCKAR
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBUN501
Plasmid#50582PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistanceDepositorInsertszCas9D10A
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSExpressionMutationCas9D10A nickase, was derived from the zCas9 and …PromoterAtU6-26p and Ubi1pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Triple ORF LoxP ifgMosaic Donor (LH208)
Plasmid#99625PurposeTo clone the 3 ORFs for Cre dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/Lox, Mouse Targeting, and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHSN501
Plasmid#50589PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertszCas9D10A
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSExpressionMutationCas9D10A nickase, was derived from the zCas9 and …Promoter2×35Sp and AtU6-26pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S2-RAB_EKARev
Plasmid#160726PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.DepositorInsertS2-RAB_EKARev
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-RAB_EKARev
Plasmid#160727PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.DepositorInsertS3-RAB_EKARev
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LoxP1-His-H2B-Cherry-2A (SO290)
Plasmid#99616PurposeTo clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassetteDepositorInsert6xHis-H2B-Cherry
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-jRCaMP1a
Plasmid#160728PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-jRCaMP1a
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
4xnr UAS ifgMosaic Donor vector (IG230)
Plasmid#99631PurposeTo clone the 3 ORFs for Gal4 dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only