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pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM)

Plasmid

#191642

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

ΔTM

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)

Plasmid

#191643

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Linker region sequence shuffled

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadH)-Pdadh-DadhABC(A2)

Plasmid

#191644

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-24xopto-TetO-no01

Plasmid

#174887

Purpose

Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

24xopto-TetO

Use

Southern blot probe

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-Rheb(5A)

Plasmid

#192439

Purpose

Encodes mCherry-tagged Rheb lacking residues 38-42.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb(C181S) (RHEB Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Rheb amino acids 38-42 deleted.

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT002

Plasmid

#182712

Purpose

aTc inducible dCasRx-IF1

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

3x(GGGS)-Escherichia Coli Initiation Factor 1

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT004

Plasmid

#182714

Purpose

Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

CasRx crRNA cloning backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_W403A_6xH

Plasmid

#185712

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed W403 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-miRFP670-eDHFR(69K6)

Plasmid

#178852

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-mNG-eDHFR(69K6)

Plasmid

#178853

Purpose

Mammalian expression of mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-eDHFR(69K6)-iRFP713

Plasmid

#178855

Purpose

Mammalian expression of eDHFR(69K6)-iRFP713

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

eDHFR(69K6)-iRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP670-eDHFR(69K6)

Plasmid

#178859

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-L36AL39A_T

Plasmid

#147604

Purpose

Insect Expression of Dm4E-T-L36AL39A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-L36AL39A (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations (H628R and N659S) compar…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup-Y342AL347A_O

Plasmid

#147103

Purpose

Insect Expression of DmCup-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup-Y342AL347A (cup Fly)

Use

Tags

Expression

Insect

Mutation

Y342A, L347A, I350L, P418S, K1045N

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET-UTX(420-633)-H6

Plasmid

#183773

Purpose

Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

UTX

Use

Tags

6 His tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-510-Y10AL15A-V5His6_D

Plasmid

#146152

Purpose

Insect Expression of Dm4E-T_1-510-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510-Y10AL15A (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-Rab7

Plasmid

#174459

Purpose

Express iRFP with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with late endosome-targeting sequence

Use

Tags

small GTPase (Rab7)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pG.Lantern-INS-SM50-GFP

Plasmid

#135594

Purpose

Propogation of Insulator-SM50 Promoter-GFP DNA for microinjection to visualize Insulator-GFP promoter activity in S. purpuratus

Depositor

Mamiko Yajima, Koji Akasaka

Article

Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

Insert

INS-SM50 promoter (SM50 )

Use

In vitro transcription (mrna synthesis)

Tags

Green Lantern GFP

Expression

Mutation

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC018.AAV

Plasmid

#171146

Purpose

Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

hAAVS1 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC030.306

Plasmid

#171151

Purpose

Mevalonate production pathway with J306 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J306 scRNA
J3-BBa_J23117-mvaES

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J3-BBa_J23117

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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