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pscALPS-SIV-AGI vpx

Plasmid

#115815

Purpose

Encodes codon optimized 3xFLAG-SIVAGI Vpx (HM803690) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGI Vpx (HM803690)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-DRL vpx

Plasmid

#115814

Purpose

Encodes codon optimized 3xFLAG-SIVDRL Vpx (KM378563) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDRL Vpx (KM378563)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MNE vpx

Plasmid

#115813

Purpose

Encodes codon optimized 3xFLAG-SIVMNE Vpx (U79412) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMNE Vpx (U79412)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RsACR_995-EYFP

Plasmid

#103772

Purpose

Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin RsACR_995

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GcACR_457-EYFP

Plasmid

#103137

Purpose

Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin GcACR_457

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pVRa17_1691

Plasmid

#49672

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF17_1691

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRc35_3582

Plasmid

#49750

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS35_3582

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin F146A

Plasmid

#45973

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin F146A (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_741-EYFP

Plasmid

#103961

Purpose

Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_741

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pVRa37_2513

Plasmid

#49696

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF37_2513

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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GTL-ir

Plasmid

#81102

Purpose

Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

tdiRFP

Use

Cre/Lox

Tags

tdiRFP

Expression

Yeast

Mutation

codon optimized second iRFP to avoid internal rec…

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pVRa07_980

Plasmid

#49659

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF07_980

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVRc16_3622

Plasmid

#49738

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS16_3622

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa37_3390

Plasmid

#49697

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF37_3390

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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hSRAP.HMG6.wt.Dlink.a1-236

Plasmid

#49813

Purpose

expression vector for MBP-SRAP(1-236) fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein (SRA1 Human)

Use

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa16_973

Plasmid

#49669

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF16_973

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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SpRY

Plasmid

#198544

Purpose

Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpRY

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Sc++

Plasmid

#198545

Purpose

Expresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

Sc++

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpG

Plasmid

#198543

Purpose

Expresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpG

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin

Plasmid

#227367

Purpose

For bacterial expression of parkin

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression.

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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