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Plasmid#153238PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only
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JPF0464
Plasmid#124035PurposeEncodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pEF1a-iRFP713_non targeting sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0465
Plasmid#124036PurposeEncodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pEF1a-iRFP713_mScarlet sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0466
Plasmid#124037PurposeEncodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pEF1a-iRFP713_tagBFP sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0468
Plasmid#124038PurposeEncodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pEF1a-iRFP713_mScarletsgRNA_tagBFP sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLux02
Plasmid#78282PurposemRFP1 expressed behind an AHL-inducible constructDepositorInsertAHL inducible mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUAS5x:GFPNLS
UseZebrafish expressionTagsExpressionMutationPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUASM1:GFPNLS-SV40pA
Plasmid#155123PurposeTol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUASM1:GFPNLS
UseZebrafish expressionTagsExpressionMutationPromoterQUASM1-E1bAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLux01
Plasmid#78281PurposemRFP1 expressed behind an AHL-inducible constructDepositorInsertAHL inducible mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTet01
Plasmid#78283PurposemRFP1 expressed behind an ATc-inducible constructDepositorInsertTet inducible mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCX-SponGee-Kras
Plasmid#134777Purposemammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signalingDepositorInsertSponGee-Kras
UseTagsFLAG and mRFPExpressionMammalianMutationPromoterCAGAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
JPF0419d
Plasmid#124020PurposeEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vectorDepositorInsertPB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUASM1:mScarletNLS-SV40pA
Plasmid#155124PurposeTol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUASM1:mScarletNLS
UseZebrafish expressionTagsExpressionMutationPromoterQUASM1-E1bAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
JPF0419v
Plasmid#124021PurposeEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vectorDepositorInsertPB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTet02
Plasmid#78284PurposemRFP1 expressed behind an ATc-inducible constructDepositorInsertTet inducible mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
px552-sg-gria1-HT
Plasmid#187652PurposeContains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.DepositorInsertsHaloTag donor sequence
miRFP670
UseAAV and CRISPRTagsN/AExpressionMammalianMutationPromoterSynapsinAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H2B-μDpTT
Plasmid#182328PurposeHistone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertH2B-μDronpa-TagRFP-T (H2BC3 Human)
UseTagsFull-length human histone H2BExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR LAT/Zap70 iLID-Drop
Plasmid#171038PurposeLight-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-labeled.DepositorInsertLAT (LAT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-acta1:QFGal4-SV40pA
Plasmid#155121PurposeTol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertacta1:QFGal4
UseZebrafish expressionTagsExpressionMutationPromoteracta1Available sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pseudojanin (PJ)
Plasmid#37999DepositorUseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only