Showing: 2641 - 2660 of 6451 results
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Plasmid#67149PurposepSG5 containing N terminally HA tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterSV40, T7AvailabilityAcademic Institutions and Nonprofits only -
pSG5 N-RFP SADBs
Plasmid#67153PurposepSG5 containing N-terminally RFP tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsRFPExpressionMammalianMutationPromoterSV40AvailabilityAcademic Institutions and Nonprofits only -
pBH-UAS-secA5-YFP
Plasmid#32359PurposeLive imaging of apoptotic cells in zebrafishDepositorInsertsecreted human annexin 5 (ANXA5 Human)
UseZebrafish expressionTagsExpressionMutationPromoterUASAvailabilityAcademic Institutions and Nonprofits only -
p1977 (pUC19-PTDH3-PTEF1)
Plasmid#78230PurposeExample plasmid containing a bidirectional promoter of pTDH3 and pTEF1DepositorInsertBidirectional promoters pTDH3-pTEF1
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#78231PurposeExpresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistanceDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSG4K5
Plasmid#74492PurposeFor cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119AvailabilityAcademic Institutions and Nonprofits only -
CIB81-pmGFP
Plasmid#75366PurposeExpresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on endDepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsprenylated EGFPExpressionMammalianMutationresidues 1-81 of CIB1PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
CIB1-CreC(N1)
Plasmid#75367PurposeExpresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinaseDepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsCre DNA recombinase, residues 106-343ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
CRY2(L348F)-CreN
Plasmid#75368PurposeExpresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinaseDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsCre recombinase, residues 19-104ExpressionMammalianMutationL348FPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Gal4BD-CRY2(535)
Plasmid#75369PurposeYeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGal4BDExpressionYeastMutationPromoterADH1AvailabilityAcademic Institutions and Nonprofits only -
CRY2PHR(W349R)-mCherry
Plasmid#75370PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherryDepositorInsertCRY2 residues 1-498 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationW349RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
CRY2(535, L348F)-mCherry
Plasmid#75372PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherryDepositorInsertCRY2 residues 1-535 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationL348FPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Ctcf-Halo-mAID donor
Plasmid#113103PurposeDonor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequenceDepositorInsertc terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Synthetic, Mouse)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
QSOX1-luciferase
Plasmid#113352Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MyosinVb-Ctail
Plasmid#110170PurposeDominant negative effect by competing with MyosinVb C-terminal tail interactionsDepositorInsertMyosin Vb (Myo5b Rat)
UseTagsEGFPExpressionMammalianMutationamino acids 1262-1670PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsA
Plasmid#107558PurposePlasmid for titrating synthesis of FtsA with doxycyclineDepositorInsertftsA
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsB
Plasmid#107559PurposePlasmid for titrating synthesis of FtsB with doxycyclineDepositorInsertftsB
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailabilityAcademic Institutions and Nonprofits only -
pONSY-mCherry
Plasmid#111874PurposeThis plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.DepositorInsertmCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailabilityAcademic Institutions and Nonprofits only -
pONSY-Venus
Plasmid#111875PurposeThis plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.DepositorInsertVenus
UseCapsaspora owczarzakiTagsVenusExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailabilityAcademic Institutions and Nonprofits only -
pONSY-Lifeact:mCherry
Plasmid#111876PurposeThis plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.DepositorInsertLifeact fused to mCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailabilityAcademic Institutions and Nonprofits only
Showing: 2641 - 2660 of 6451 results