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pET30(a)-AtNUDX23

Plasmid

#175021

Purpose

Purification of AtNUDX23 enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

AtNUDX23 (NUDX23 Mustard Weed)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSFV3

Plasmid

#92072

Purpose

Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Type

Empty backbone

Use

Empty expression plasmid, for run-off transcripti…

Tags

Expression

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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mWasabi-C1

Plasmid

#54743

Purpose

Localization: C1 Cloning Vector, Excitation: 493, Emission: 509

Depositor

Michael Davidson, Robert Campbell

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Use

Tags

mWasabi

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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DiLiCre 2.0

Plasmid

#198752

Purpose

A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

Depositor

Jacco van Rheenen

Article

Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

Insert

Cre-PhoCI-ERT2-ERT2

Use

Lentiviral

Tags

Cre, kept in a photocleavable site, followed by 2…

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GV

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

Plasmid

#132405

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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VE-cad-eGFP Donor plasmid

Plasmid

#92309

Purpose

Knock eGFP into human VE-cad locus

Depositor

Sean Palecek

Article

Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

Insert

VEcad Homologous Recombination Arms (CDH5 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

about 30 a.a. truncated at end of protein

Promoter

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Apple-53BP1trunc

Plasmid

#69531

Purpose

Fluorescent reporter for DNA damage

Depositor

Ralph Weissleder

Article

Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

Apple fluorescent protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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gag-COM-pol

Plasmid

#211373

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-COM-pol

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-PEmax for IVT

Plasmid

#178113

Purpose

Template for in vitro transcription of PEmax

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF0373

Plasmid

#141594

Purpose

Lentiviral vector for overexpressing the NFYA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFYA (NFYA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mPiezo2-CMV-Sport6

Plasmid

#81073

Purpose

mouse Piezo2

Depositor

Ardem Patapoutian

Article

Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

Insert

Piezo2 (please see depositor comments below) (Piezo2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 ALK4 T206D

Plasmid

#80880

Purpose

mammalian expression of ALK4 T206D

Depositor

Peter ten Dijke, Aristidis Moustakas

Article

ten Dijke et al Science. 1994 Apr 1;264(5155):101-4.

Insert

ALK4 (ACVR1B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T206D

Promoter

CMV

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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mTOR_2 shRNA

Plasmid

#1856

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

mTOR (MTOR Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

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PT7lacO-RF1-His6

Plasmid

#224372

Purpose

Express RF1 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

RF1 (prfA E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-Q74

Plasmid

#40262

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q74 (HTT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV OMM-FKBP-V5-sTurboID (N)

Plasmid

#153006

Purpose

Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

OMM (Tom20)-sTurboID (N) (L73)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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OMM-V5-LOV-Turbo

Plasmid

#199665

Purpose

expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

AKAP1 transmembrane domain and V5

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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mGFP-10-sREACh-N3

Plasmid

#21947

Depositor

Ryohei Yasuda

Article

Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

Insert

mGFP-10-sREACh

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pBeloBac11loxP2272

Plasmid

#60342

Purpose

This is a BAC vector that contains both wt and mutant loxP sites.

Depositor

Jon Coren

Article

Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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