Addgene

We narrowed to 11,260 results for: pcDNA

Showing: 2621 - 2640 of 11260 results

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pcDNA-HA-hTRAF3 1-448

Plasmid

#44033

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Use

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 1-448; V274D and S393G

Promoter

Available since

April 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A-mGFP

Plasmid

#62633

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

mGFP inserted in M3-M4 loop

Promoter

CMV

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 SV8

Plasmid

#184011

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Wild type cDNA

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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mcherry Gamma 9 ---pcDNA3.1

Plasmid

#64203

Purpose

G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

G protein Gamma 9 (GNGT2 Human)

Use

Tags

6xHis and mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-LKB1(KD)

Plasmid

#84640

Purpose

mCherry-tagged, LKB1(K78I); kinase-dead mutant

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(K78I) (STK11 Synthetic, Human)

Use

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Lysine 78 mutated to Isoleucine; kinase dead

Promoter

CMV

Available since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG_withTEVsite

Plasmid

#124939

Purpose

Expresion of RGG_GFP-RGG

Depositor

Benjamin Schuster, Daniel Hammer, Matthew Good

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

TEV site between RGG and EGFP

Promoter

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1MKK7B2

Plasmid

#19731

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

Jnk1a1MKK7b2 (Map2k7, MAPK8 Mouse, Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1his p66shc S36A

Plasmid

#10973

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

S36A

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-Akt-STOPS

Plasmid

#175006

Purpose

Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-PNGase-Strep-HA

Plasmid

#113490

Purpose

Expression of human PNGase with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

PNGase (NGLY1 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2sh-E138A

Plasmid

#97068

Purpose

Expresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Glutamic Acid 138 to Alanine

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-AKARev(T/A)

Plasmid

#118479

Purpose

Negative-control mutant for RAB-AKARev biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev(T/A)

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPH1

Plasmid

#19453

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPH1 (HSPH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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S100B_pcDNA6.2/EmGFP-Bsd

Plasmid

#176952

Purpose

Mammalian expression vector encoding S100B and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

S100B (S100B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG_FBXL7 (R310H)

Plasmid

#171849

Purpose

pcDNA3-FLAG_FBXL7 (R310H)

Depositor

Michele Pagano

Article

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

F-box and leucine rich repeat protein 7 (FBXL7 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Arginine 310 to Histidine

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK2 K235R

Plasmid

#80872

Purpose

mammalian expression of ALK2 K235R

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK2 (ACVR1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K235R

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1(-) TRIM12B

Plasmid

#87079

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12B (Trim5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Axin1

Plasmid

#123463

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Axin 1 (Axin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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mP58.FL1-pCDNA3

Plasmid

#21883

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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