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pCDNA3-TEX264-SIM1_mut-cFlag

Plasmid

#220205

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-METTL14-WT

Plasmid

#141112

Purpose

Overexpression of WT METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

HMETTL14 (METTL14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

Plasmid

#159853

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mNeon(PATC)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLBC0060

Plasmid

#172783

Purpose

Level 0 module containing NT2_cYFP3XHA

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238) 3XHA tag

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0061

Plasmid

#172784

Purpose

Level 0 module containing CT_cYFP3XHA

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238) 3XHA tag

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHyg-His6

Plasmid

#212776

Purpose

C-terminal PCR-based tagging with His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLBC0131

Plasmid

#172793

Purpose

Level 0 module containing CDS2ns_deltamPTAC5

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature PTAC5 with C-ter delection (41-252), Arabidopsis thaliana

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPD47_52

Plasmid

#1446

Depositor

Andrew Fire

Type

Empty backbone

Use

Tags

Sp6p, T7p, OriLam>>, OriLam<<, KanRN,…

Expression

Worm

Mutation

Promoter

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pJOG021

Plasmid

#105420

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

hpt (hygromycin phosphotransferase)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG351

Plasmid

#105421

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

nptII (neomycin phosphotransferase II)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-KRAS4B(G12V)

Plasmid

#35633

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pQCXIP GFP-MOSPD2 RD/LD

Plasmid

#186468

Purpose

Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

R404D/L406D (mutant unable to bind FFAT motifs)

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-ER-KRAS(G12V)

Plasmid

#35635

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

ER

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-KRAS4A(G12V)

Plasmid

#35634

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4A(G12V) (KRAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-FRT

Plasmid

#113398

Purpose

expresses gRNA for Cas9 FRT targetting

Depositor

Jan Michiels

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

exo, beta, gam, sgRNA-FRT

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO10-pcDNA3.1-

Plasmid

#52508

Purpose

expresses human FbxO10 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO10 (FBXO10 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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CAGGS-SAFB-NLS-3XFLAG-V5

Plasmid

#196089

Purpose

Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-SIM1_mut-cGFP

Plasmid

#220212

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV/TetO

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMK188

Plasmid

#226659

Purpose

pCB2.4 - pBR322 shuttle vector that replicates in E. coli and Synechococcus sp. PCC 7002, contains HIP1 site

Depositor

David Nielsen

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS3

Plasmid

#17645

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS3 (POLY Hepatitis C virus)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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