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pQE60 P126G wgMDH

Plasmid

#204260

Purpose

Bacterial expression of P126G wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

P126G

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P123G wgMDH

Plasmid

#204256

Purpose

Bacterial expression of P123G wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

P123G

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 D193E wgMDH

Plasmid

#204269

Purpose

Bacterial expression of D193E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

D193E

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R130E wgMDH

Plasmid

#204267

Purpose

Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R130E

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P123F wgMDH

Plasmid

#204255

Purpose

Bacterial expression of P123F wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

P123F

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-hArfaptin1

Plasmid

#206402

Purpose

Mammalian expression of hArfaptin1

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin1 (ARFIP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-hArfaptin2

Plasmid

#206403

Purpose

Mammalian expression of hArfaptin2

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin2 (ARFIP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A D197,199N

Plasmid

#195704

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9 with D1…

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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MPRA Empty Vector

Plasmid

#204865

Purpose

Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

Depositor

Philipp Maass

Article

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYG205

Plasmid

#200836

Purpose

Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives

Depositor

Boris Görke

Article

Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

Insert

rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P-BAD

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pHcRed-CLCa

Plasmid

#196911

Purpose

visualization of clathrin-coated vesicle in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

Insert

Clathrin-Coated light chain

Use

Tags

HcRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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NeuA.pJL1

Plasmid

#199115

Purpose

In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)

Depositor

Michael Jewett

Article

Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

Insert

N-acylneuraminate cytodiylyltransfease (NeuA)

Use

Tags

Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-DmeIF4G_578-650_Z

Plasmid

#148183

Purpose

Bacterial Expression of DmeIF4G_578-650

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4G_578-650 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmeIF4G_578-650-GB1_Z

Plasmid

#148154

Purpose

Bacterial Expression of DmeIF4G_578-650

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4G_578-650 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-LLFWKtoAAAAA_Z

Plasmid

#148156

Purpose

Insect Expression of Dm4E-T-LLFWKtoAAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-LLFWKtoAAAAA (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation L127S, annotated as EST, …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_1-417-DmeIF4G_686-1666_T

Plasmid

#147584

Purpose

Insect Expression of DmCup_1-417-DmeIF4G_686-1666

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup_1-417-DmeIF4G_686-1666 (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T_1-510-Y10AL15A-V5His6_T

Plasmid

#147600

Purpose

Insect Expression of Dm4E-T_1-510-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510-Y10AL15A (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSD

Plasmid

#195208

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

S162/339/340D

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 PDZ1/2*

Plasmid

#195211

Purpose

Mammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutant

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Dual PDZ1/2 GYGF>GYAA motif mutant

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-3JB1R+4

Plasmid

#190841

Purpose

For mammalian expression of 3JB1R+4, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

3JB1R_AP3+4bp (A Dual-Specificity aptamer targeting OGT and GFP)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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