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pCDNA4TO-3XFLAG Halo WT nsp1

Plasmid

#175412

Purpose

Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 nsp1 (ORF1ab CoV2)

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-human NPM1 wt

Plasmid

#160692

Purpose

Mammalian expression of FLAG-tagged NPM1

Depositor

Charles Sherr

Article

Bertwistle et al Mol Cell Biol. 2004 Feb . 24(3):985-96.

Insert

NPM1 (NPM1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAN19-3xFLAG-ccvTIR1-NOSt

Plasmid

#108546

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Expression

Mutation

Changed F79 to G

Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+(Zeo)-FLAG-muGFP

Plasmid

#166726

Purpose

Expresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cells

Depositor

Daniel Scott

Article

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Insert

muGFP

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Flag-DHHS

Plasmid

#85813

Purpose

To rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 C134S mutant (Zdhhc5 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PARP1-Flag-His-WT

Plasmid

#111572

Purpose

Expresses full length PARP1 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Use

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-FLAG-dTomato-centrin-1

Plasmid

#73332

Purpose

Lentiviral vector encoding centrin-1 with an N-terminal FLAG and dTomato tag

Depositor

Manuel Thery

Insert

centrin-1 (CETN1 Human)

Use

Lentiviral

Tags

FLAG and dTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST DICER

Plasmid

#19881

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Dicer 1, ribonuclease type III (DICER1 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-IDH1-R132H

Plasmid

#62907

Purpose

Expresses R132 mutant IDH1 in mammalian cells

Depositor

Yue Xiong

Article

Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

Insert

IDH1 (IDH1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deleted Arg132 (R132)

Promoter

Available since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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nLuc-GLP-1R-His-FLAG

Plasmid

#124831

Purpose

Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells

Depositor

Sam Gellman

Article

Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. d

Insert

GLP1R (GLP1R Synthetic, Human)

Use

Luciferase

Tags

FLAG, His6, and NanoLuc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HA-FLAG RBP2 H483A

Plasmid

#14801

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Changed His 483 to Ala, R46W

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCDH-3xFLAG-GFP-puroR

Plasmid

#167463

Purpose

3rd-gen lentiviral expression of FLAG-GFP in mammalian cells

Depositor

Steven Artandi

Article

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-Flag-NRBP1

Plasmid

#48197

Purpose

Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1)

Depositor

Joan Conaway, Ronald Conaway

Article

Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

Insert

NRBP1 (NRBP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pHR-flag-GFP-Ecad

Plasmid

#205188

Purpose

This vector encodes of a synCAM (Ecad) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM Ecad and GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(D669A)

Plasmid

#220461

Purpose

Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Aspartate 669 to alanine

Promoter

CMV

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag-humanTRAF6 (1093)

Plasmid

#66929

Purpose

Mammalian Expression of TRAF6

Depositor

Michael Karin

Insert

TRAF6 (TRAF6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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1105-Desmoglein2-FLAG-eGFP

Plasmid

#36989

Depositor

Kathleen Green

Article

Klessner et al Mol Biol Cell. 2009 Jan;20(1):328-37. Ep

Insert

Dsg2 (DSG2 Human)

Use

Tags

FLAG and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA GNSTM-3-Flag-10-Lamp2b-HA

Plasmid

#71293

Purpose

Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Use

Tags

FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-Flag-PALB2

Plasmid

#71114

Purpose

mammalian expession of FLAG-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-FLAG-MTOR-S2215Y

Plasmid

#69013

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S2215Y

Promoter

CMV

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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