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Plasmid#159918PurposeMutagenesis of Drd2DepositorInsertDrd2 (Drd2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHdCas9
Plasmid#137080PurposeExpression of Himar-dCas9 in bacteriaDepositorInsertHimar-dCas9
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1
Plasmid#159905PurposeMutagenesis of Slc32a1DepositorInsertSlc32a1 (Slc32a1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAT74
Plasmid#204296PurposepJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-Cas9-H2B-mCherry
Plasmid#175570PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationXbaI site added upstream of U6 promotor. G to C …PromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
spCas9-BlastR (pCBhCas9-BlastR)
Plasmid#71489PurposeExpresses SpCas9 and Blasticidin resistanceDepositorInsertspCas9
UseTagsExpressionMutationPromoterCBhAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pATF4.1.0-gDNA
Plasmid#113772PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4DepositorInsertATF4 (ATF4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
EC70195
Plasmid#209456PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU3Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgDrd1
Plasmid#159917PurposeMutagenesis of Drd1DepositorInsertDrd1 (Drd1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMLS291
Plasmid#73724PurposeSapTrap mCherry + Cbr-unc-119 donor plasmidDepositorInsertmCherry + Cbr-unc-119
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCD061
Plasmid#113315PurposeW108 scRNA.b1DepositorInsertW108 scRNA.b1_MS2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX-sgRNA-BfuAI-2k
Plasmid#112915PurposeEmpty sgRNA expression plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-p300-Hygro
Plasmid#104411Purposetransient expression of dCas9-p300 fusion proteinDepositorInsertp300 (EP300 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p(U6b-BsaI-gRNA)
Plasmid#65956PurposeTribolium U6b promoter with BsaI cloning site to insert guide RNA sequenceDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterU6Available sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLS134
Plasmid#73714PurposeSapTrap destination vector for building sgRNA expression vectors.DepositorInsertpU6::sgRNA
UseCRISPRTagsExpressionWormMutationSapI insertion sitePromoterC. elegans U6 snRNA pol III promoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pV1090
Plasmid#111427PurposegRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)DepositorInsertsgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS252
Plasmid#73720PurposeSapTrap GFP + Cbr-unc-119 donor plasmidDepositorInsertGFP + Cbr-unc-119
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS256
Plasmid#73715PurposeSapTrap destination vector for building combined sgRNA expression + repair template vectorsDepositorInsertpU6::sgRNA
UseCRISPRTagsExpressionWormMutationSapI insertion sitePromoterC. elegans U6 snRNA pol III promoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only