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pcDNA3.1 CMV NAPA-A BGH

Plasmid

#92277

Purpose

FRET probe; extracellular membrane tethered eGFP

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Astrocyte

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-tagRFP-Mad2

Plasmid

#114021

Purpose

Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

tagRFP-Mad2 (MAD2L1) (MAD2L1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-JAK1-VSV

Plasmid

#139356

Purpose

Expression of JAK1 protein

Depositor

Sandra Pellegrini

Article

Richter et al J Biol Chem. 1998 Sep 18;273(38):24723-9

Insert

JAK1 cDNA with 300nt of 5'UTR (JAK1 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2V678F-VSV

Plasmid

#139349

Purpose

Expression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)

Depositor

Sandra Pellegrini

Article

Gakovic et al J Biol Chem. 2008 Jul 4;283(27):18522-9.

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

V362F-V678F

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pCMV-7.1

Plasmid

#47948

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

Insert

3xFLAG-dCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A (catalyticall…

Promoter

CMV

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-LC3B WT

Plasmid

#123114

Purpose

Expresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Use

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5B-TGFbeta receptor I K232R

Plasmid

#11763

Depositor

Jeff Wrana

Article

Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

Insert

TGFbeta receptor I (TGFBR1 Human)

Use

Tags

6xHIS

Expression

Mammalian

Mutation

K232R: kinase dead

Promoter

CMV

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pPRIME-CMV-GFP-FF3

Plasmid

#11663

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with CMV promoter controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-eBFP2-NLS-pA

Plasmid

#183800

Purpose

AAV vector to express nuclear localized eBFP2 from CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eBFP2-NLS

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DHFR.cHSF1

Plasmid

#134739

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(PGK/CMV)

Plasmid

#172330

Purpose

LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Mutation

Promoter

PGK/CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRcCMV Cep215 (Nigg CW493)

Plasmid

#41152

Depositor

Erich Nigg

Article

Graser et al J Cell Sci. 2007 Dec 15;120(Pt 24):4321-

Insert

Cep215 (CDK5RAP2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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CMV-VPR-S-L1-dCjCas9 MiniCAFE

Plasmid

#169910

Purpose

Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

MiniCAFE

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D8A, HNH-truncation (Δ495–609 aa) in CjCas9

Promoter

CMV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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piggyBac CMV-GFP-FRT

Plasmid

#92078

Purpose

production of transgenic animal and recombination using flipase

Depositor

Jae Yong Han

Article

Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.

Insert

CMV-eGFP and TK-Neo between FRT3 and FRT

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-F2A-T2A

Plasmid

#62622

Purpose

AdEasy Shuttle vector modified for multicistronic expression. Contains T2A and F2A

Depositor

Zheng-Xu Wang

Insert

MCS with F2A and T2A

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-Dre-T2A-TagRFP

Plasmid

#167968

Purpose

Transient Dre expression

Depositor

Emma Rawlins

Article

Sun et al Elife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

Dre recombinase

Use

Tags

TagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-MEK1_fusion

Plasmid

#39194

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
3XFlag pCMV5-TOPO TAZ WT

Plasmid

#24809

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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CMV-mEGFP(A206K)-paCaMKII

Plasmid

#165438

Purpose

Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII

Use

Tags

mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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