Addgene

We narrowed to 14,286 results for: jun

Showing: 2581 - 2600 of 14286 results

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pJH857

Plasmid

#172175

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH202

Plasmid

#172150

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

gIII(P29*); 32A pylS variant

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH223

Plasmid

#172151

Purpose

pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/lac (pylBCD 34B_sub-pop5); LacI

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E178K, E251D) PylD(A252V)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH224

Plasmid

#172152

Purpose

pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/lac (pylBCD 40C_sub-pop6); LacI

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH463

Plasmid

#172155

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 36A_sub-pop1); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E122K, E178K, G188R)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH573

Plasmid

#172157

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (Untagged pylBCD); AraC

Use

Tags

Expression

Bacterial

Mutation

Codon-optimized Methanosarcina acetivorans pylBCD

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH574

Plasmid

#172158

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 36A_sub-pop2); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K) PylC(E316D)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH575

Plasmid

#172159

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop3); AraC

Use

Tags

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH576

Plasmid

#172160

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop4); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K, E251D) PylD(A252V)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH19

Plasmid

#172141

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

gIII(P29*)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH60

Plasmid

#172142

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.CD

Use

Tags

Expression

Bacterial

Mutation

PylCD partial operon (PylB deleted to assess impa…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH77

Plasmid

#172145

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.3TAG, luxAB; Plpp pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

gIII(P29*, P83*, Y184*)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH123

Plasmid

#172146

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.B.(SD4)CD

Use

Tags

Expression

Bacterial

Mutation

PylBCD operon (RBS inserted for PylC)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH126

Plasmid

#172147

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.SUMO-B.(SD4).CD

Use

Tags

Expression

Bacterial

Mutation

PylBCD operon (SUMO tag inserted for PylB; RBS in…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#2

Plasmid

#163395

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#1

Plasmid

#163394

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#3

Plasmid

#163396

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Use

Tags

8-His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1C-YFP-shSCR

Plasmid

#139647

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

shSCR

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

See Depositor comments

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBL-2

Plasmid

#162769

Purpose

Minimal backbone with AmpR

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Use

Synthetic Biology; Molecular cloning

Tags

Expression

Mutation

Promoter

no

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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