-
Plasmid#78624PurposeEncodes sgRNA2.0 targeting 158nt upstream of TSSDepositorInsertsgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA:Asip
Plasmid#60968PurposeExpression vector of rat Asip guide RNADepositorInsertAgouti signaling protein gRNA (Asip Rat)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSaGuide
Plasmid#64710PurposeCloning and expression of Staphylococcus aureus guide RNADepositorInsertStaphylococcus aureus guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin
Plasmid#115519PurposeTo construct multiple sgRNA expression vector for CRISPRDepositorInsertccdB-sgRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSmart-Nm-sgRNA-BbsI
Plasmid#49157PurposePlasmid for cloning spacer into sgRNA for NmCas9DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pORFE0005
Plasmid#112073PurposeAtDeadCAS9 D10A H840A without stop codon module CDS1nsDepositorInsertAtDeadCAS9 D10A H840A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLK88 - Edit-directing plasmid base
Plasmid#98814PurposeEdit-directing plasmid baseDepositorInsertCAN1.y gRNA
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMK
Plasmid#62818PurposeBacterial constitutive S. pyogenes Cas9 expression plasmidDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_Bfl-1.1
Plasmid#85536PurposeInducible expression of guide RNA (Bfl-1.1) with fluorescent GFP reporterDepositorInsertBfl1.1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_Bfl-1.2
Plasmid#85537PurposeInducible expression of guide RNA (Bfl-1.2) with fluorescent GFP reporterDepositorInsertBfl1.2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST
Plasmid#203812PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRR1
Plasmid#166096PurposeGal inducible expression of Cas9 WTDepositorInsertWT spCas9
UseCRISPRTagsExpressionYeastMutationPromoterGal-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD5
Plasmid#73914Purposemultiplex gRNA plasmidDepositorInsertdU6-3:tRNA:gRNA:tRNA:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAW016-2
Plasmid#85613PurposeCas9-tracrRNA integration vector for Bacillus subtilisDepositorInsertcas9-tracrRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW014-2
Plasmid#85612PurposeMulti-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilisDepositorInsertugtP-gRNA.P395T
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW019-2
Plasmid#85614PurposeInducible dCas9 integration vector for Bacillus subtilisDepositorInsertdcas9
UseTagsExpressionBacterialMutationPromoterPxylA (B. megaterium)Available sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1-TagBFP2
Plasmid#124773PurposeLentiviral plasmid to co-express a guide RNA and TagBFP2DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEA186
Plasmid#187874PurposeEmpty pVS1-based INTEGRATE vector with mCherry cargoDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL2310
Plasmid#187875PurposeEmpty pVS1-based INTEGRATE vector with loxP cargoDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJUNB.1.0-gDNA
Plasmid#112409PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNBDepositorInsertJUNB (JUNB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only