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Plasmid#39854DepositorInsertEmi1 (FBXO5 Human)
UseIn vitro transcriptionTagsVenusExpressionMutationPromoterT7Available sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
talin1-head
Plasmid#182996Purposeexpression of talin1 head wt in mammalian cellsDepositorInserttalin1 head (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT9624-BEAR-cloning
Plasmid#162986PurposeBEAR target cloning plasmid with split EGFPDepositorInsertexchangeable cassette
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
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phNFL-Q332P
Plasmid#132599PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutationDepositorInsertNefl (Nefl Human)
UseTagsExpressionMammalianMutation995A>C;Gln332ProPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
phNFL-E396K
Plasmid#132595PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation1186G>A; Glu396LysPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
SNAP-EF(N)-60nmSAH-GFP(1-10)
Plasmid#61663Purpose2x-30nm SAH scaffold with EF Hand and split GFP attachment sitesDepositorInsertSNAP-EF(N)-60nmSAH-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-Flag-dTSC2 WT
Plasmid#111807PurposeInsect Expression of dTSC2DepositorInsertdTSC2 (gig Fly)
UseTags3xFlagExpressionInsectMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
SNAP-EF(N)-5nmSAH-GFP(1-10)
Plasmid#61659Purpose5nm SAH scaffold with EF Hand and split GFP attachment sitesDepositorInsertSNAP-EF(N)-5nmSAH-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt(R25C)
Plasmid#39532DepositorInsertAkt(R25C) (Akt1 Mouse)
UseTags6G linker, EGFP, and HAExpressionMammalianMutationR25CPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P440L
Plasmid#132596PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation1319C>T; Pro440LeuPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-CD4-mCherry-HA-FRB
Plasmid#100740PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertCD4
UseTagsFRB, HA, Myc, and mCherryExpressionMammalianMutationEngineered to remove large part of extracellular …PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-NGFP-mAID
Plasmid#140605PurposeExpression of OsTIR1 and GFP-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGBDU-Atg17
Plasmid#121394PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECFP-Cdc42-Y40C
Plasmid#105295PurposeControl for FRET biosensor analysis - cannot bind downstream effectorsDepositorInsertCdc42 (CDC42 Human)
UseTagsCFPExpressionMammalianMutationY40CPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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SNAP-EF(N)-30nmSAH-GFP(1-10)
Plasmid#61662Purpose30nm SAH scaffold with EF Hand and split GFP attachment sitesDepositorInsertSNAP-EF(N)-30nmSAH-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-NmScarlet-mAID
Plasmid#140618PurposeExpression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEos2-CD36-C-10
Plasmid#57357PurposeLocalization: Membrane, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertCD36 (CD36 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits