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pCMV-R-Kras

Plasmid

#180572

Purpose

ddRFP sensor of Kras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Kras

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pC4-RHE-PAS (FRB-PAS)

Plasmid

#80002

Purpose

miRFP PAS domain fused to FRB

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FRB

Use

Tags

PAS domain of miRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)

Plasmid

#80004

Purpose

miRFP709 GAF domain fused to FKBP and NLS

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FKBP

Use

Tags

GAF domain of miRFP709 and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Cdc42

Plasmid

#180578

Purpose

ddRFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Cdc42

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pC4EN-F1-mGAF670 (FKBP-mGAF670)

Plasmid

#80003

Purpose

miRFP670 GAF domain fused to FKBP

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FKBP

Use

Tags

GAF domain of miRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pWA PAS-E BAD K-GAFm

Plasmid

#39866

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Inserts

iRFP protein PAS domain
iRFP protein GAF domain (mutated)

Use

Tags

E coil and K coil

Expression

Bacterial

Mutation

F165Y, D232Y, W308R

Promoter

arabinose inducible and rhamnose inducible

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pTSTEP

Plasmid

#72334

Purpose

Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)

Depositor

Avital Rodal

Article

Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

Insert

RRS-TagRFPT-p10-[Golic+] RRS-EGFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTSTEP-SNAPf

Plasmid

#72335

Purpose

Tissue-specifically swappable (C-terminal TagRFPT-to-SNAPf) genome tagging plasmid with Golic+ selection cassette, for fully transgenic gene targeting (Drosophila melanogaster)

Depositor

Avital Rodal

Article

Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

Insert

RRS-TagRFPT-p10-[Golic+] RRS-SNAPf

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBW842_pCAG-pExpr-FP-DECODER

Plasmid

#87551

Purpose

Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP, EGFP, iRFP720, mRuby2

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-R-Hras

Plasmid

#180579

Purpose

ddRFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Hras

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pIM23

Plasmid

#74643

Purpose

Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Use

Tags

Expression

Yeast

Mutation

Promoter

MET25

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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MTK3b_005

Plasmid

#123786

Purpose

Encodes iRFP670 as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

iRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3b_011

Plasmid

#123792

Purpose

Encodes iRFP713 as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

iRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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IDP379

Plasmid

#87006

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

postLib-mRFP-4xboxB

Use

Synthetic Biology

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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p21-Reporter

Plasmid

#52432

Purpose

DNA damage inducible Red Fluorescent Protein for mammalian expression

Depositor

Joel Voldman

Article

Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

Insert

p21-p53 Tandem Response Element Promoter

Use

Tags

TurboRFP

Expression

Mammalian

Mutation

Promoter

13X repeated p21-p53 response element

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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RA-NES

Plasmid

#61019

Purpose

ddRFP A-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A

Use

Tags

HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGEP163

Plasmid

#137881

Purpose

PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

mRFP-T2A-BlasticidinR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTcI39-switch

Plasmid

#195557

Purpose

Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mRFP1
mWasabi
TcI39

Use

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCX-SponGee

Plasmid

#134775

Purpose

mammalian expression of SponGee, a buffer of cGMP that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

SponGee

Use

Tags

FLAG and mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pPPC017.109

Plasmid

#171145

Purpose

Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J109 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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