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pQC hKGA.S286A V5-His IRES G418

Plasmid

#110391

Purpose

γ-Retroviral transfer vector for expressing glutminase isoform KGA (S286A), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Insert

KGA glutaminase (GLS Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Mutation

S286A

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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shFGF2#1

Plasmid

#157667

Purpose

Tet inducible knockdown of FGF2

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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gh135

Plasmid

#106802

Purpose

expression of gRNA targeting EXT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

EXT1 (EXT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh115

Plasmid

#106785

Purpose

expression of gRNA targeting GCNT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GCNT1 (GCNT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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CYTIP_PDZ_1

Plasmid

#103930

Purpose

Protein expression and purification of PSCDBP PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

CYTIP (CYTIP Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CD2AP-3/3

Plasmid

#91508

Purpose

Protein expression and purification of human SH3 domain construct CD2AP-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CD2AP-3/3 (CD2AP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF38-1/1

Plasmid

#91373

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF38-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF38-1/1 (ARHGEF38 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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MYO3B gRNA (BRDN0001145051)

Plasmid

#75568

Purpose

3rd generation lentiviral gRNA plasmid targeting human MYO3B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MYO3B (MYO3B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MYO3B gRNA (BRDN0001145615)

Plasmid

#75570

Purpose

3rd generation lentiviral gRNA plasmid targeting human MYO3B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MYO3B (MYO3B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MYO3B gRNA (BRDN0001147808)

Plasmid

#75571

Purpose

3rd generation lentiviral gRNA plasmid targeting human MYO3B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MYO3B (MYO3B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MYO3B gRNA (BRDN0001145445)

Plasmid

#75569

Purpose

3rd generation lentiviral gRNA plasmid targeting human MYO3B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MYO3B (MYO3B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-F656W

Plasmid

#72546

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

F656W

Promoter

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-I654Q

Plasmid

#72545

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

I654Q

Promoter

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-L630W

Plasmid

#72543

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L630W

Promoter

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Casp8-mod

Plasmid

#48171

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> ATT) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of CASP8 (CASP8 Human)

Use

Luciferase

Tags

Expression

Mutation

G/T MUTATION IN ATG OF UORF

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Hsdl2-mod

Plasmid

#48180

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of HSDL2 (Hsdl2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

A/T MUTATION IN ATG OF UORF

Promoter

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Hsdl2

Plasmid

#48179

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of HSDL2 (Hsdl2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2c-E471Q

Plasmid

#45704

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

E471Q

Promoter

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only
pRLCon1288-1666

Plasmid

#31448

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRLCon183-221_850-1207

Plasmid

#31449

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragments (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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