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Plasmid#153528PurposeExpresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.DepositorInsertHes7 (Hes7 Mouse)
UseTagsAchillesExpressionMammalianMutationPromotermouse Hes7 promoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 hSlp2-a
Plasmid#40056DepositorInserthSlp2-a (SYTL2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-H2B-GCaMP6s
Plasmid#59530PurposeExpressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTransposable elementTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)
Plasmid#164193PurposeFreeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)DepositorInsertsCamPARI2
Human α Tubulin
UseAAVTags6 His tag and NESExpressionMammalianMutationF391W-L398VPromoterSynAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-Sec8
Plasmid#53758PurposepEGFP-C3 plasmid that expresses Sec8DepositorInsertSec8 (EXOC4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVNP3.0-ABE8e
Plasmid#206882PurposeExpresses FLAG-tagged ABE8e fused to Gag through a linker sequenceDepositorInsertABE8e
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS HSPA8
Plasmid#19541DepositorInsertheat shock 70kDa protein 8 (HSPA8 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-hSOX17FNV
Plasmid#206384PurposeExpresses human SOX17FNV in mammalian cells, for lentivirus generation.DepositorInsertSOX17FNV (SOX17 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH-nCas9-PPE-V2
Plasmid#170131PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1, OsU3Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA
Plasmid#55201PurposePlasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVNP3.0-PEmaxRH
Plasmid#206884PurposeExpresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequenceDepositorInsertPEMax Delta RNAseH
UseTagsFLAGExpressionMutationPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Basigin-isoform2-CD4d3+4-bio
Plasmid#73129PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bioDepositorInsertBasigin isoform 2 (BSG Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA
Plasmid#55202PurposePlasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pF712-pET28a-Hs-delta44-GCDH-NHis wt
Plasmid#85113Purposeexpression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta44) GCDH (GCDH Human)
UseTags6xHisExpressionBacterialMutationsilent SNP: G1041TPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a_FLI1_P2A_Hygro_Barcode
Plasmid#120437PurposeBarcoded lentiviral vector to express FLI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertFLI1 (FLI1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_FOXA2_P2A_Hygro_Barcode
Plasmid#120439PurposeBarcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertFOXA2 (FOXA2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g1 (BB22)
Plasmid#139457PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g2 (BB23)
Plasmid#139458PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A8
Plasmid#131989PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A8 (SLC22A8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR-53BP1 1-1972 28A
Plasmid#53447PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGatewayTagsExpressionMutationI914T mutation; All 28 SQ/TQ motifs in N-term of …PromoterAvailable sinceDec. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits