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Plasmid#90033PurposeTo express His-tagged RvLEAM in bacteriaDepositorInsertRvLEAM
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLGB36
Plasmid#135621PurposeSuicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselectionDepositorInsertbfe3
UseBacteroides suicide vector with inducible counter…Tagssignal sequence for periplasmic targetingExpressionMutationPromoterBacteroides promoter regualted by TetR transcript…Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
Plasmid#207180PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a PromoterDepositorInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCSC-NLS-RFP
Plasmid#221813PurposeLentiviral vector expressing red fluorescent protein (RFP) fused with nuclear localization sequence (NLS)DepositorInsertNLS-mCherry
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_R249S
Plasmid#22935DepositorInserttumor protein 53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationR249S MutantPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Synthetic_Human_H2A.1
Plasmid#42634DepositorInsertHuman H2A.1 (H2AC13 Human, Synthetic)
UseTagsUntaggedExpressionBacterialMutationCodon OptimizedPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
CAG-GFP/cre
Plasmid#49054PurposeRetroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinaseDepositorInsertGFP/Cre
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-GDH
Plasmid#108954Purposeheterologous expression of GDH of B. megaterium in E. coliDepositorInsertGlucose dehydrogenase IV (GDH IV)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-cMyc
Plasmid#46970Purposelentiviral expression of c-MycDepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab7 DN
Plasmid#12662DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsDsRed and HAExpressionMammalianMutationT22NPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
TFORF0257
Plasmid#141560PurposeLentiviral vector for overexpressing the TCF4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA_Cloning Vector
Plasmid#41824PurposeAn empty gRNA expression vector, used to create a gRNA to a specific sequence.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsAflII site for Gibson AssemblyExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
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FLAG-Stx17
Plasmid#45911DepositorInsertSyntaxin 17 (STX17 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
myc-Rictor corrected
Plasmid#11367PurposeMammalian expression of myc tagged human RICTORDepositorInsertRictor (RICTOR Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDHt/sk-CE
Plasmid#92128PurposeExpression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reeseiDepositorInsertCas9-eGFP
UseCRISPR; Trichoderma reesei expressionTagsEGFPExpressionMutationPromoterPcbh2Available sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB551
Plasmid#74881PurposeGateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag
Plasmid#132399Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
V1-STING
Plasmid#124263PurposeN-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorInsertTMEM173 (STING1 Human)
UseTagsVenus1ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-null
Plasmid#200182PurposeExpresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 siteDepositorInsertAAV9 Cap w/ nonsense mutation at VR8
UseAAVTagsExpressionMammalianMutationReplaced amino acids 586-592 in Cap with nonsense…PromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgRNA
Plasmid#124844PurposeVector for Cre-dependent expression of SaCas9DepositorInsertSaCas9, gRNA scaffold
UseAAV, CRISPR, and Mouse TargetingTagsNLS and NLS-3xHAExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP670
Plasmid#136571PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertH2B-emiRFP670
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
NL4-3 mCherry Luciferase
Plasmid#44965PurposeHIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.DepositorInsertmCherry, Luciferase, IRES
UseLentiviralTagsLuciferase, IRES and mCherry with D212GExpressionMutationS34C mutation in NefPromoterAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-GFP-wt-lamin A
Plasmid#17662DepositorInsertGFP-lamin A (LMNA Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET15b-mISG15(GG)
Plasmid#12448DepositorInsertC-terminal processed form of ISG15
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
PGK-EGFP-FKBPF36V
Plasmid#199765Purposeplasmid for targeting dTAG into the AAVS1 locusDepositorInsertGFP-dTAG
UseTagsGFP and dTAGExpressionMammalian and WormMutationPromoterhPGKAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pDEST17-UbE2L6
Plasmid#15797DepositorInsertUbiquitin conjugating enzyme UbE2L6 (UBE2L6 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-VP64
Plasmid#47107PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cellsDepositorInsertdCas9-VP64
UseCRISPRTagsFlag, HA, SV40 NLS, and VP64ExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only