Addgene

We narrowed to 781 results for: vill

Showing: 241 - 260 of 781 results

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MYC sgRNA5

Plasmid

#100557

Purpose

Expresses MYC sgRNA5. Target sequence GAGAGGCAGAGGGAGCGAGC

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

MYC sgRNA5

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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MYC sgRNA4

Plasmid

#100556

Purpose

Expresses MYC sgRNA4. Target sequence: GTAATTCCAGCGAGAGGCAG

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

MYC sgRNA4

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLXMA L1A

Plasmid

#153347

Purpose

Expression vector for plants with medium-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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hCas9

Plasmid

#41815

Purpose

Expresses human codon optimized Cas9 nuclease for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T2

Plasmid

#41818

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pOO2-GW

Plasmid

#135097

Purpose

Gateway vector for expression of proteins in Xenopus oocytes

Depositor

Wolf Frommer

Article

Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

Type

Empty backbone

Use

Bacterial expression vector for generating sp6 tr…

Tags

Expression

Mutation

Promoter

SP6

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T1

Plasmid

#41817

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-Myo19

Plasmid

#127612

Purpose

GFP-tagged Myosin-19

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Myo19

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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BFP-Rab5CA (Q79L)

Plasmid

#205705

Purpose

Mammalian expression of BFP-tagged mutant Rab5CA

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

RAB5A (RAB5A Human)

Use

Tags

Expression

Mammalian

Mutation

Q79L

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T1

Plasmid

#41819

Purpose

Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T2

Plasmid

#41820

Purpose

Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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ciCas9_pcDNA5

Plasmid

#100550

Purpose

Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-pOTC-3H-GFP

Plasmid

#205725

Purpose

Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

pOTC-3H-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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hPRL3_1-169

Plasmid

#209796

Purpose

Bacterial expression of human PRL-3 without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2004 Mar 19. 279(12):11882-

Insert

PRL-3 (PTP4A3 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 WT

Plasmid

#195213

Purpose

Mammalian expression vector for GFP tagged NHERF1.

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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786 pCSmAKT2Acherry

Plasmid

#31167

Depositor

Nathan Lawson

Article

Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

Insert

Akt

Use

Expression vector

Tags

Myr and mCherry

Expression

Mutation

membrane tethered Akt

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
TtGNN-GFP-empty

Plasmid

#106380

Purpose

dox inducible expression of GFP with neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pME-Fv2E-flt4um200-ns

Plasmid

#67696

Purpose

middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4um200

Use

Tags

HA

Expression

Mutation

um200, no stop codon; N1056S

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pME-6MTzactMap2k2b

Plasmid

#67710

Purpose

Middle entry clone

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

activated Map2k2b (map2k2b Zebrafish)

Use

Tags

Expression

Mutation

S221D, S225D

Promoter

none

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pXOOY

Plasmid

#196449

Purpose

A dual vector for expression of membrane proteins in S. cerevisiae and X. laevis

Depositor

Per Amstrup Pedersen

Article

Vold et al PLoS One. 2023 Feb 21;18(2):e0281868. do

Type

Empty backbone

Use

Expression in xenopus laevis

Tags

Expression

Yeast

Mutation

Promoter

yeast CYC-GAL and T7 promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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