Addgene

We narrowed to 585 results for: pBR322

Showing: 241 - 260 of 585 results

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pJH806_pJH_LldR_Pcon-sfGFP.1TAG

Plasmid

#163737

Purpose

Constitutive expression of green fluorescent protein encoding one stop codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TAG

Use

Tags

Expression

Bacterial

Mutation

gfp.N39TAG

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH28_pBad_AraC_Para-sfGFP.3TAG

Plasmid

#163723

Purpose

Arabinose-inducible expression of green fluorescent protein encoding three amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.3TAG

Use

Tags

Expression

Bacterial

Mutation

gfp.N39TAG,N135TAG,Y151TAG

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH626_pJH_LldR_PlldR-sfGFP.1TAG

Plasmid

#163725

Purpose

Lactate-inducible expression of green fluorescent protein encoding one amber codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TAG

Use

Tags

Expression

Bacterial

Mutation

gfp.N39TAG

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH627_pJH_LldR_PlldR-sfGFP.2TAG

Plasmid

#163726

Purpose

Lactate-inducible expression of green fluorescent protein encoding two amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TAG

Use

Tags

Expression

Bacterial

Mutation

gfp.N39TAG,Y135TAG

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH628_pJH_LldR_PlldR-sfGFP.3TAG

Plasmid

#163727

Purpose

Lactate-inducible expression of green fluorescent protein encoding three amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.3TAG

Use

Tags

Expression

Bacterial

Mutation

gfp.N39TAG,N135TAG,Y151TAG

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH721_pJH_LldR_PlldR-sfGFP.20TAG

Plasmid

#163729

Purpose

Lactate-inducible expression of green fluorescent protein encoding twenty amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.20TAG

Use

Tags

Expression

Bacterial

Mutation

gfp with all 20 leucine codons mutated to amber

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH726_pJH_LldR_PlldR-sfGFP.15TAG

Plasmid

#163730

Purpose

Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.15TAG

Use

Tags

Expression

Bacterial

Mutation

gfp with first 15 leucine codons mutated to amber

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH727_pJH_LldR_PlldR-sfGFP.10TAG

Plasmid

#163731

Purpose

Lactate-inducible expression of green fluorescent protein encoding ten amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.10TAG

Use

Tags

Expression

Bacterial

Mutation

gfp with first 10 leucine codons mutated to amber

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH774_pJH_LldR_PlldR-sfGFP.1opal

Plasmid

#163733

Purpose

Lactate-inducible expression of green fluorescent protein encoding one opal codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TGA

Use

Tags

Expression

Bacterial

Mutation

gfp with first leucine codon mutated to amber

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH775_pJH_LldR_PlldR-sfGFP.2opal

Plasmid

#163734

Purpose

Lactate-inducible expression of green fluorescent protein encoding two opal codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TGA

Use

Tags

Expression

Bacterial

Mutation

gfp with first two leucine codons mutated to amber

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH578

Plasmid

#172162

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2_v2); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH767

Plasmid

#172172

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WT]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH857

Plasmid

#172175

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH223

Plasmid

#172151

Purpose

pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/lac (pylBCD 34B_sub-pop5); LacI

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E178K, E251D) PylD(A252V)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH224

Plasmid

#172152

Purpose

pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/lac (pylBCD 40C_sub-pop6); LacI

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH463

Plasmid

#172155

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 36A_sub-pop1); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E122K, E178K, G188R)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH573

Plasmid

#172157

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (Untagged pylBCD); AraC

Use

Tags

Expression

Bacterial

Mutation

Codon-optimized Methanosarcina acetivorans pylBCD

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH574

Plasmid

#172158

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 36A_sub-pop2); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K) PylC(E316D)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH575

Plasmid

#172159

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop3); AraC

Use

Tags

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH576

Plasmid

#172160

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop4); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K, E251D) PylD(A252V)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge