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C071

Plasmid

#174343

Purpose

Template for making Nluc mRNA reporter with pA29.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

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C093

Plasmid

#174344

Purpose

Template for making Nluc mRNA reporter with pA63.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLSU1/t35s[TMV]:NLuc-I{AGO-405}:NosT

Plasmid

#212182

Purpose

This binary vector expresses Nanoluciferase (Nluc) containing the Argonaut (AGO) intron (405 bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR

Depositor

Wayne Curtis

Insert

NanoLuc luciferase-I{AGO-405}

Use

Tags

Expression

Plant

Mutation

AGO intron added at bp 405, eliminates expression…

Promoter

truncated 35sCaMV

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0133

Plasmid

#154595

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

LucN (NanoLuc)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBH111

Plasmid

#215778

Purpose

NBU2 backbone with anti-LacI(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

anti-LacI(YQR)
Nanoluc

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Bt1311 and P(O1)

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH101

Plasmid

#215772

Purpose

NBU2 backbone with LacI(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(YQR)
Nanoluc

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BT1311 and P(O1)

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH102

Plasmid

#215773

Purpose

NBU2 backbone with LacI(TAN) and P(tta)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(TAN)
Nanoluc

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Bt1311 and P(tta)

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH103

Plasmid

#215774

Purpose

NBU2 backbone with LacI(KSL) and P(agg)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(KSL)
Nanoluc

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Bt1311 and P(agg)

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH104

Plasmid

#215775

Purpose

NBU2 backbone with LacI(HQN) and P(ttg)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(HQN)
Nanoluc

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Bt1311 and P(ttg)

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH105

Plasmid

#215776

Purpose

NBU2 backbone with LacI(GKR) and P(gac)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(GKR)
Nanoluc

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Bt1311 and P(gac)

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH106

Plasmid

#215777

Purpose

NBU2 backbone with RbsR(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

RbsR(YQR)
Nanoluc

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Bt1311 and P(O1)

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH116

Plasmid

#215779

Purpose

NBU2 backbone with anti-RbsR(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

anti-RbsR(YQR)
Nanoluc

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Bt1311 and P(O1)

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-nLuc

Plasmid

#199755

Purpose

Control construct of elongation reporter to quantify elongation of YFP

Depositor

Brian Zid

Article

Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

Insert

Nanoluc

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0197

Plasmid

#154654

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

NOS Nanoluc expression cassette [NOS::TMV::LucN::Flag::nosT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYCα2rKN0009

Plasmid

#154616

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_066 Nanoluc expression cassette [MinSyn_066::LucN::Flag::nosT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYCα2rKN0010

Plasmid

#154617

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_064 Nanoluc expression cassette [MinSyn_064::LucN::Flag::nosT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0091

Plasmid

#154618

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_064 Nanoluc expression cassette [MinSyn_064::LucN::Flag::g7T]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPKKα2KN0092

Plasmid

#154619

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_065 Nanoluc expression cassette [MinSyn_065::LucN::Flag::g7T]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPKKα2KN0093

Plasmid

#154620

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_066 Nanoluc expression cassette [MinSyn_066::LucN::Flag::g7T]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPKKα2KN0100

Plasmid

#154621

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_067 Nanoluc expression cassette [MinSyn_067::LucN::Flag::g7T]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge