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MG15-gal (mut p53 binding sites)

Plasmid

#16447

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (mutant) (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pg-dsRedXP-HIF-2alpha

Plasmid

#87209

Purpose

N-terminally dsRedXP-tagged human HIF-2a/EPAS

Depositor

Violaine Sée

Article

Taylor et al Open Biol. 2016 Sep;6(9). pii: 160195.

Insert

HIF-2a (EPAS1 Human)

Use

Tags

dsRedXP

Expression

Mammalian

Mutation

Q868E

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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D3 clone (p21/WAF1 promoter)

Plasmid

#16452

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 promoter (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
PG13-luc (wt p53 binding sites)

Plasmid

#16442

Purpose

Luciferase reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2

Plasmid

#132775

Purpose

Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-GFP

Plasmid

#132776

Purpose

Prime editing in mammalian cells with co-translational GFP expression

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2-P2A-GFP

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189925

Purpose

AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SauriABE8e

Use

AAV

Tags

Expression

Mutation

SauriCas9 D15A

Promoter

EFS

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189922

Purpose

AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e

Use

AAV

Tags

Expression

Mutation

SaCas9 D10A

Promoter

EFS

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1-SIO-stGtACR2-FusionRed

Plasmid

#105677

Purpose

Expresses a soma-targeted GtACR2 in Cre-expressing neurons

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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hSynapsin-PE2-Cterminal-lenti

Plasmid

#135956

Purpose

Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) C-terminal

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

MMLV RT(D200N, T306K, W313F, T330P, L603W)

Promoter

hSynapsin

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

Plasmid

#135955

Purpose

Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) N-terminal

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CKIIa-stGtACR2-FusionRed

Plasmid

#105669

Purpose

Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has Service

AAV Retrograde and AAV1

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-PuroDTK.Neo

Plasmid

#72307

Purpose

PiggyBac Cassette contains puromycin and TK genes

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Insert

Puromycin-DTK-neo

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG promoter

Available since

Feb. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Neo-PuroDTK

Plasmid

#84036

Purpose

PiggyBac cassette contains Neomycin, puromycin and TK genes

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG promoter

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP-WAF1-ES

Plasmid

#16449

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 stop codon mut (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

stop codon at amino acid 49 instead of tryptophan…

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
PX330-PTEN

Plasmid

#227440

Purpose

Cas9 expression and a gRNA targeting Exon 6 of mouse PTEN

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

spCas9 and gRNA for mouse PTEN (Pten )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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PGL4.11-COL1A1WT

Plasmid

#230915

Purpose

Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter (COL1A1 Human)

Use

Luciferase

Tags

luciferase - Luc2p

Expression

Bacterial and Mammalian

Mutation

Promoter

COL1A1

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189923

Purpose

AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e V106W

Use

AAV

Tags

Expression

Mutation

nSaCas9 KKH, TadA ABE8e V106W

Promoter

EFS

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

Plasmid

#189924

Purpose

AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e V106W

Use

AAV

Tags

Expression

Mutation

SaCas9 D10A, TadA ABE8e V106W

Promoter

EFS

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMP71-hCD155

Plasmid

#118630

Purpose

human CD155 coding sequence subcloned into pMP71 retroviral plasmid

Depositor

Sébastien Walchli

Article

Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

Insert

CD155 (PVR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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