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Plasmid#73829PurposeExpress human Plekhm1 C-part in insect cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTags6xHis and FlagExpressionInsectMutationcloned C-terminal part of human Plekhm1 (aa 493- …PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plekhm1-N terminal pFastHFTb
Plasmid#73828PurposeExpress human Plekhm1 N-part in insect cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTags6xHis and FlagExpressionInsectMutationcloned N- terminal part of human Plekhm1 (aa 1- 4…PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-moesina TAL1
Plasmid#41831DepositorInsertmoesin a (msna Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-moesina TAL2
Plasmid#41832DepositorInsertmoesin a (msna Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
ninaE-stops m3-myc
Plasmid#26855DepositorInsertSlow Termination of Phototransduction (stops Fly)
UseTagsmycExpressionInsectMutationAmino acids 296-299 (SLRH in wild-type) are mutat…PromoterAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-mCardinal-Sh_ble
Plasmid#120334PurposeBacterial mCardinal promoter trapDepositorInsertmCardinal
UseTagsExpressionBacterialMutationPromoter(None)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab35 S22N inactive
Plasmid#47426DepositorInsertRab35 S22N (RAB35 Human)
UseTagsGFPExpressionMammalianMutationS22N inactivePromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE C-terminal
Plasmid#137178PurposeAAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSynapsin-PE2-Cterminal-lenti
Plasmid#135956PurposeNeuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3bDepositorInsertPrime editor v2 (PE2) C-terminal
UseLentiviralTagsExpressionMammalianMutationMMLV RT(D200N, T306K, W313F, T330P, L603W)PromoterhSynapsinAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE C-terminal
Plasmid#137176PurposeAAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVTagsExpressionMutationPromoterCbhAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
BirA(R118G)-HA destination vector
Plasmid#53581PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)DepositorTypeEmpty backboneUseTagsBirA(R118G) and HA tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE N-terminal
Plasmid#137177PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoterDepositorInsertv5 AAV-ABE N-terminal
UseAAVTagsExpressionMutationCas9 D10APromoterCbhAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + terminators reporter plasmid
Plasmid#156456PurposeA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.DepositorInsertsNeoR/KanR
TcR
UseTagsExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7 and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-neo-cterminal-3HA
Plasmid#102643PurposeTo generate three copies of the HA epitope tag on cDNAs.DepositorTypeEmpty backboneUseTags3x HA (YPYDVPDYA)ExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc mTOR kinase dead
Plasmid#8482DepositorInsertmTOR (Mtor Rat)
UseTagsmycExpressionMammalianMutationD2357E V2364IPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE N-terminal
Plasmid#137175PurposeAAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE N-terminal; U6-protospacer
UseAAVTagsExpressionMutationCas9 D10APromoterCbhAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ATR Kinase dead
Plasmid#31612DepositorInsertATR (ATR Human)
UseTagsFlagExpressionMammalianMutationK2327R (kinase dead)PromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3nls
Plasmid#157854PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertdCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dominant negative Hsp90 beta
Plasmid#22480DepositorInsertDominant negative Hsp90 (HSP90AB1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3M14nes
Plasmid#157855PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertUseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only