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Plasmid#59636PurposeProbe for Drosophila melanogaster Or7a expressionDepositorInsertOr7a (Or7a Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-7Zf-wild
Plasmid#23160DepositorInsertRibonuclease assay sub1
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEM-7Zf-deleted
Plasmid#23161DepositorInsertRibonuclease assay sub2
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEM cLrdr (CT#900)
Plasmid#13950DepositorInsertLrdr
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-GEM-GECO1
Plasmid#32461PurposeMammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertGEM-GECO
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Mef2c-tPT2A-GFP
Plasmid#111771PurposeBicistronic construct: Co-express two genes by 2A peptidesDepositorInsertMef2c-tPT2A-GFP (Mef2c Mouse)
UseCloning intermediateTagsGFPExpressionMutationPromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mClover3-MAP4-MTBD
Plasmid#171498PurposeT7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNADepositorInsertMAP4-MTBD (Map4 Mouse)
UsePgehmeTagsmClover3ExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mScarlet-MAP4-MTBD
Plasmid#171488PurposeT7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNADepositorInsertMAP4-MTBD (Map4 Mouse)
UsePgehmeTagsmScarletExpressionMutationAminoacids 658-1126PromoterT7Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-dCas13-APO1-YTH
Plasmid#204767PurposeGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)DepositorInsertdCas13-NLS-APO1-YTH-HA
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEf1alphaAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)
Plasmid#176932PurposeDonor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locusDepositorInserthGEM(1-110)_mNG_hSLBP(18-108)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGK1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9_hGEM(1-110)_hSLBP(56-108)
Plasmid#191388PurposeSpCas9 fused with geminin and SLBP degrons to improve HDR ratioDepositorInserthGEM(1-110)_hSLBP(56_108)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGem-PgpdA-Cre-TtrpC
Plasmid#168784PurposeHelper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.DepositorInsertCre
UseCre/Lox and Synthetic Biology; Expression in fila…TagsExpressionMutationPromoterPgpdAAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ GEMIN6 myc HIS
Plasmid#71659PurposeMammalian expression of GEMIN6DepositorInsertGEMIN6 (GEMIN6 Human)
UseTagsHIS and mycExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM-GFP-URA3-GFP
Plasmid#72606PurposeTemplate for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeastDepositorInsertsGFP (full)
URA3
GFP (partial)
Optional Linker
UseTagsExpressionMutationIncludes full 819 bp coding sequence of URA3, 435…PromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Map7-1333-end
Plasmid#46070DepositorInsertMap7-1333-end (Map7 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF-miRFP670v1-hGem(1/110)
Plasmid#80006PurposehGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)DepositorInserthGeminin (1/110) (GMNN Human)
UseLentiviralTagsmiRFP670v1ExpressionMutationPromoterEF-1 alphaAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterENO1pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLeo-A-a2-GEMS(JAK-STAT)
Plasmid#209110PurposeTransient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)DepositorInsertA-a2-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-miRFP-MAP4-MTBD
Plasmid#171487PurposeT7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNADepositorInsertMAP4-MTBD (Map4 Mouse)
UsePgehmeTagsmiRFPExpressionMutationAminoacids 658-1126PromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21ΔRING-Box
Plasmid#105520PurposeT7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationdeletion of RING domain (aa124-462)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only