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FLAG-KIF5AR280H
Plasmid#166957PurposeExpresses FLAG-tagged KIF5A R280H protein in pcDNA3.1DepositorInsertKIF5A-R280H (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationKIF5A-R280HPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXD4L6
Plasmid#153157PurposeExpression of Flag tagged human FOXD4L6 in mammalian cellsDepositorInserthuman FOXD4L6 (FOXD4L6 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE99Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXD4L1
Plasmid#153154PurposeExpression of Flag tagged human FOXD4L1in mammalian cellsDepositorInserthuman FOXD4L1 (FOXD4L1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE96Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXD4L3
Plasmid#153155PurposeExpression of Flag tagged human FOXD4L3 in mammalian cellsDepositorInserthuman FOXD4L3 (FOXD4L3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE97Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-hCCDC11
Plasmid#122993PurposeExpresses Flag-tagged human CCDC11 in mammalian cellsDepositorInserthuman CCDC11 (CFAP53 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5
Plasmid#125234PurposeExpresses SPARK oβ2AR transmembrane component in mammalian cellsDepositorInsertgLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5
UseAAVTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
Plasmid#187611PurposeSplit luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassetteDepositorInsertsWWE domain with C-terminal nLuc
WWE domain with C-terminal cLuc
UseLentiviralTagsFLAG, HA, Split luciferase (cLuc), Split lucifera…ExpressionMammalianMutationPromoterEF1AAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
Plasmid#187612PurposeSplit luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassetteDepositorInsertsWWE domain (R163A) with C-terminal nLuc
WWE domain with C-terminal cLuc
UseLentiviralTagsFLAG, HA, Split luciferase (cLuc), Split lucifera…ExpressionMammalianMutationArg163AlaPromoterEF1AAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_FLAG_MTA3
Plasmid#31696DepositorInsertMetastasis associated protein 3 (MTA3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_APEX_FLAG_Nt
Plasmid#194068Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsV5-APEX-FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_APEX_FLAG_Ct
Plasmid#194069Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-APEX-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
Plasmid#92099PurposeDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnTagsExpressionMammalianMutationPromoterTet-On 3G BidirectionalAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET283xFlagPP7CPHis
Plasmid#28174DepositorInsertPP7 coat protein optimized
UseTags6xhistidine and triple FLAG peptideExpressionBacterialMutationC68A,C73APromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_TurboID_FLAG_Nt
Plasmid#194072Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsV5-TurboID-FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMVhTRIM65_3Flag
Plasmid#53966PurposeHuman TRIM65 mammalian expression vector with 3xFLAG epitope at c-terminus.DepositorInsertTRIM65 (TRIM65 Human)
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_3xFlagEzh2 WT
Plasmid#173717PurposeWT EZH2 expression plasmid with 3XFLAG tagDepositorInsertEZH2 (EZH2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_R248W
Plasmid#183114PurposeExpresses flag-tagged p53 with R248W mutation in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged arginine 248 to tryptophanPromoterEF1aAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_DESTFLAGHA
Plasmid#26361DepositorTypeEmpty backboneUseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only